Objective To investigate the effects of subthreshold micropulse yellow laser (577 nm) on vascular endothelial growth factor (VEGF),nerve growth factor (NGF) and Chemerin expressions in retina of early stage diabetic rats.Methods A total of 40 Brown Norway rats were treated with streptozocin (65 mg · kg-1) to establish the diabetic model.20 diabetic BN rats' right eyes were received subthreshold micropulse yellow laser (577 run) therapy after 2 weeks.The left eyes were used as control group.At 3 days,7 days,14 days,28 days after laser therapy,5 BN rats were randomly chosen to perform RT-PCR and Weston-blot.The expressions of mRNA and protein of VEGF,NGF and Chemerin were analyzed.Results The expression of VEGF mRNA and protein increased in control group at 3 days,7 days,14 days and 28 days (all P ＜ 0.05).Compared with the control group,VEGF mRNA and protein decreased in the subthreshold micropulse yellow laser (577 nm) group (all P ＜ 0.05).The expression of NGF mRNA and protein decreased in the control group at 3 days,7 days,14 days and 28 days (all P ＜ 0.05),however,the difference was not statistically significant between 3 days and 7 days(P ＞0.05).Compared with control group,NGF mRNA and protein increased in the subthreshold micropulse yellow laser (577 nm) group (all P ＜ 0.05),with maximum expression at 14 days.The expression of chemerin mRNA and protein increased at 3 days,7 days,14 days and 28 days in the control group (all P ＜0.05).Compared with the control group,chemerin mRNA and protein decreased in the subthreshold micropulse yellow laser (577 urn) group (all P ＜ 0.05).Conclusion Subthreshold micropulse yellow laser (577 urn) can suppress VEGF,Chemerin expression and upregulate NGF expression in early stage diabetic rats.%目的 观察阈值下577 nm微脉冲激光光凝对早期糖尿病大鼠视网膜血管内皮生长因子(vascular endothelial growth factor,VEGF)、神经生长因子(nerve growth factor,NGF)和趋化素(Chemerin)表达的影响.方法采用65 mg·kg-1链脲佐菌素对40只Brown Norway大鼠建立糖尿病大鼠模型,选取20只造模成功者为实验鼠,造模后2周右眼采用IQ577 nm激光行阈值下微脉冲激光处理为微脉冲激光组,左眼不做任何处理为对照组.在微脉冲激光光凝后3d、7d、14 d、28 d随机各选取5只大鼠,应用实时荧光定量PCR和Western blot方法检测大鼠双眼视网膜VEGF、NGF和Chemerin表达情况.结果 对照组VEGF mRNA和蛋白在3d、7d、14 d、28 d表达均增加,差异均有统计学意义(均为P＜0.05);微脉冲激光组VEGF mRNA和蛋白在3d、7d、14 d、28 d均较对照组下降,差异均有统计学意义(均为P＜0.05).对照组3d、7d、14 d、28 d NGF mRNA和蛋白均逐渐下降(均为P＜0.05),7 d NGF比3d下降,但差异无统计学意义(P＞0.05);微脉冲激光组NGF mRNA和蛋白在3d、7d、14 d、28 d均较对照组上升,且在14 d达到峰值,差异均有统计学意义(均为P＜0.05).对照组Chemerin mRNA和蛋白在3d、7d、14 d、28 d表达均增加,差异均有统计学意义(均为P ＜0.05);微脉冲激光组Chemerin mRNA和蛋白在3d、7d、14 d、28 d均较对照组下降,差异均有统计学意义(均为P＜0.05).结论 阈值下577 nm微脉冲激光光凝可降低早期糖尿病大鼠VEGF、Chemerin表达,上调NGF表达.
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