目的 采用蒸发光散射检测器(ELSD)测定麦冬中麦冬皂苷D、D'的含量.方法 Waters symeteryC18色谱柱(4.6mm×150 mm,5μm),水-四氢呋喃-乙腈(52:3:45)等度洗脱,流速1.0mL·min-1,柱温30℃,增益值10,压力2.5Pa,飘移管温度65℃.结果 麦冬皂苷D和麦冬皂苷D’分别在48.60 - 972μg·mL-1,24.75 ～495μg·mL-1范围内具有良好的线性关系,平均回收率(n=6)分别为101.7％(RSD=2.69％)和97.7％(RSD =3.25％).结论 该方法快速、准确,可用于麦冬药材中麦冬皂苷D和麦冬皂苷D’的含量测定.%Objective To determinate of ophiopogonin D and ophiopogonin D' from Ophiopogon japonicus by ELSD-HPLC. Methods ELSD-HPLC conditions were as the follows Waters Symmetry C18 column (4.6 mm ×250 mm, 5μ.m) by gradient elution with water, tetrahydrofuran and acetonitrile (52:3 :45), the flow rate 1.0 mL · min-1, the column temperature maintained at 30 ℃ , the gain 10, the pressure 2.5 pa, and the draft temperature 65℃. Results The calibration curve showed a good linearity with the range of 48. 60 ～972 μg · mL-1 for ophiopogonin D and 24.75 ～495μg · mL-1 for ophiopogonin D', respectively. The recovery (n =6) was 101.7% (RSD = 2.69% ) and 97.7% (RSD =3.25% ), respectively. Conclusion This method was simple and accurate and could be used for the determination of ophiopogonin D and ophiopogonin D' from Ophiopogon japonicus.
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