目的 建立高效液相色谱法测定大麦苗提取物中β-胡萝卜素的含量测定方法.方法 色谱柱为Kromasil C18(5 μm,250 mm×4.6 mm);流动相:甲醇-三氯甲烷-正己烷(50:35:15);检测波长:448 mm,柱温:30℃.结果 β-胡萝卜素的回收率为100.4%;线性范围为0.03952～0.7904 μg.结论 结果准确,重复性好,可用于该产品的质量控制.%Objective To establish an HPLC method for the determination of β-carotene in Barley Grass extract. Methods HPLC was used for quantitative analysis. The Kromasil C18 column(5μm,250 mm×4.6mm) was used, and mobile phase was composed of methanol- Chloroform- normal hexane ( 50:35:15 ) . Detection wavelength was set at 448 nm at 30 ℃. Results The average recovery of β-carotene was 100.4％, and the linearity was good in the range of 0.039 52～0.790 4 μg. Conclusion The result is accurate and reproducibility is good. The method can be used for the quality control of the products.
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