为研究转染血管内皮生长因子(VEGF)基因的骨髓间充质干细胞(BMSCs)复合多孔CPC构建的组织工程化骨对骨缺损的修复作用,用脂质体介导质粒pIRES2-EGFP/VEGF165转染大鼠BMSCs,与多孔CPC复合体外构建组织工程化骨,植入大鼠颅骨缺损模型,未转染VEGF165的组织工程化骨作对照.分别在4周、12周取材,以组织学组织形态学分析骨缺损修复情况.比较两组的骨形成差异.结果转染VEGF组骨缺损的修复效果明显好于未转染组.组织形态计量学分析显示,4周时,转染组材料内新生骨面积为31.9%,未转染组为19.7%.12周时,转染组材料内新生骨面积为75.9%,未转染组仅为49.7%.差异具有统计学意义(P＜0.000 1).转染组的骨形成速率明显快于未转染组,两者分别为分别为6.18±1.62μm/d和3.56±0.93 μm/d.说明VEGF转染细胞组较对照组血供加强,骨缺损修复加速.%To study the repair effect of porous CPC with bone mesenchymal stem cells transfected with VEGF gene on bone defect, a eukaryotic expression vector pIRES2-EGFP/VEGF165 was transfected by liposome DoTAP into rat BMSCs,then seeded into porous CPC and constructed tissue engineered bone, which repaired rat skull defect. The porous CPC with BMSCs is control group. The follow up times were 4,12 weeks. Histological study were performed to observe the skull defect healing. It is results of that better ossification was observed in the transfected with VEGF experimental group compared to the single BMSCs with collagen control group including more newly formed bone tissue and higher rate of bone formation. The area of new bone formed (％) in the transfected with VEGF experimental group and the single BMSCs with collagen control group were 31.9％ and 19.7％ at 4 weeks respectively, and 75.9％, 49.7％ at 12 weeks. The rate of bone formation on the transfected with VEGF experimental group and the single BMSCs with collagen control group were (6.18 ±1.62) μm/d and (3.56 ±0.93) μm/d respectively. It is conclused that transfer of gene encoding VEGF increases repair of bone defect and provides rich blood supply for repairing.
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