以花楸树成熟合子胚胎为材料,利用室内培养皿发芽法研究外源NO供体(SNP)、NO合成抑制剂(PTIO)、脱落酸(ABA)以及乙烯受体抑制剂(NBD)对花楸树胚胎萌发和幼苗发育初期活性氧(ROS)积累的影响.结果表明:光照条件下SNP预处理可减弱PTIO或ABA对胚胎萌发的抑制作用,但不能减弱NBD处理对胚胎萌发的抑制作用.SNP预处理显著增加幼苗叶片中叶绿素含量,但这种增加效应不能被PTIO解除.PTIO抑制SNP预处理引起的幼苗叶片中过氧化氢(H2O2)含量和超氧阴离子含量的增加.SNP预处理解除PTIO对根部超氧阴离子含量的抑制作用.研究结果为阐明胚胎萌发过程中反应氮调节的(NO依赖型)休眠解除和ROS积累的关系奠定基础.%In this paper,mature zygotic embryo of Sorbus pohuashanensis were used to study effects of an exogenous nitric oxide donor (sodium nitroprusside,SNP),NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-l-oxyl-3-oxide (PTIO),abscisic acid (ABA) and 2,5-norbornadiene (NBD)-an inhibitor of the ethylene receptor on the embryo germination and reactive oxygen species (ROS) accumulation at early stage of the seedling development by the petri dish germination method indoors.The results showed that the inhibitory effect of PTIO or ABA on the embryo germination was relieved by the SNP pretreatment under light.However,the SNP pretreatment did not influence the inhibitory action of NBD to embryo germination.The SNP pretreatment increased the chlorophyll content in leaves of seedlings,which was not removed by PTIO treatment.The contents of hydrogen peroxide (H2O2) and superoxide anion in seedling leaves were increased by SNP pretreatment,which was cancelled by PTIO treatment.The PTIO treatment reduced the superoxide anion content in seedling roots,which was removed by SNP pretreatment.The results would lay the foundation for interpreting the relationships between reactive nitrogen species (RNS) mediated NO-dependent) dormancy removal and ROS (H2O2 and superoxide anion) accumulation in the embryo germination.
展开▼
