To understand the relationship between the contents of proteins and free amino acids and the infection of Beauveria bassiana. Changes in the contents of proteins and free amino acids in haemolymph of Apriona germari larvae infected by B. bassiana were detected. The result showed that the content of proteins increased to the maximum in 3 days after inoculation,indicating the proteins and enzymes could be produced for the immunologic responses,and then declined. By using the polyacrylamide gelelectrophoresis( PAGE) technique, the change in protein composition in Apriona germari larvae infected by Beauveria bassiana was analyzed. The result showed that the band number increased after inoculation. There were significant differences in the band widths and staining intensities. The results of amino acids analysis showed that there were abundant necessary amino acids for the strain' s growth in A. germari larvae bodies, thus B. bassiana showed a high virulence to A. germari larvae. The content of free amino acids decreased in 24 h after inoculation, suggesting that the B. bassiana invaded into the insect body absorbed some amino acids from the blood as nutrition. The content of free amino acids had no further changes from 24 -72 h,and then increased sharply in 96 h and 120 h probably because of gradual loss of immunoregulation, destroy of tissue and disintegration of proteins. Before dead of the infected A. germari larvae,the contents of different amino acids changed differentially,suggesting that different growth phases of the isolate needed different amino acids.%桑天牛现称桑粒肩天牛(Apriona germari),是多种林木、果树、花卉的重要蛀干害虫,也是我国危害最严重、最难防治的害虫之一.近年来,其猖獗危害已成为我国养蚕业、林业及果树种植业等经济发展的严重障碍.球孢白僵菌(Beauveria bassiana)是一类广谱性昆虫病原真菌,能侵染15目149科的700多种昆虫(St Leger,2001;Blake et al.,2002;Felipe et al.,2004).近年来,利用白僵菌防治松毛虫(Dendrolimus spp,)、玉米螟(Pyrausta nubilalis)、棉铃虫(Helicoverpa armigera)、美国白蛾(Hyphantria cunea)、天牛等农林害虫的研究和应用均取得很大进展,在控制农林害虫的严重发生和减少环境污染方面,发挥着不可替代的作用(赫英伟,2010;王滨,2003;丁德贵等,2004;张爱文等,1992;孙鲁娟等,2001;郭志红等,2001;刘洪剑等,2007;Shmazu,1995;徐均焕等,2003;林庆源,2005;刘洪剑等,2009;王云滨,2007;陆秀君等,2008).
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