以华北落叶松继代、增殖培养的不同类型的胚性及非胚性愈伤组织为材料，测定其氨基酸、糖类、酚酸、激素以及离子的含量，结果表明：氨基酸含量的差异十分明显，质地硬的非胚性愈伤组织中18种游离氨基酸含量为胚性愈伤组织的2倍以上，经ABA处理后，胚性愈伤组织中多糖及邻苯二酚含量急剧升高；三糖含量与不同基因型密切相关；乙烯释放量在非胚性愈伤组织中高于胚性愈伤组织；金属离子CO＋＋的含量，胚性愈伤组织是非胚性愈伤组织的12倍，胚性愈伤组织中铵态氮(NH+4)与硝态氮(NO-3)的比值是非胚性愈伤组织中的10倍以上；实验结果为进一步探讨针叶树体细胞胚胎发生的生理生化机理提供依据。%Based on the appearance of callus growth and behaviour (structure,color etc.)of Larix principis|Rupprechtii embryogenic callus,the physiological and biochemical background in these different kinds of embryogenic and non|embryogenic calli were analyzed.This included as well callus treated by abscisic acid (ABA).The contents of amino acids,saccharides,hydroxybenzene acid,phytohormones as well as different ions were tested.It was indicated that the average content of 18 amino acids of non|embryogenic calli were higher than embryogenic calli.Especially the contents of Arginine,Lysine and Valine in the non|embryogenic calli were 3～4 times higher as in embryogenic callus.The contents of amylose and trisaccharide have shown no different changes between embryogenic and non|embryogenic calli.The contents of amylos and O|phenylenediphthol in embryogenic calli treated by ABA was rising rapidly,18 times as the un|treated control.The correlation between trisaccharide content and different genotypes was very high.The ethylene content observed in the embryogenic callus was lower compared with the non|embryogenic callus.The Co++ content of embryogenic callus was higher than in non|embryogenic callus (12 times).The content of NH++-4 was almost double and NO+--3 was 20|fold higher in NEC.The NH++-4/NO+--3 ratio of embryogenic callus is more than 10 times that of the non|embryogenic callus.The above|mentioned changes might explain some physiological and biochemical differences between these two cell types.
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