目的：探讨 PM2．5对 RTE 大鼠气管上皮细胞的氧化应激及自噬的影响。方法：采集制备不同浓度的 PM2．5来处理 RTE 细胞，用 MTT 比色法测定细胞增值率，流式细胞术检测细胞内活性氧类（ROS）自由基生成，Western Blot 检测微管相关蛋白1轻链3 I 蛋白（LC3I）和 LC3II 表达。结果：PM2．5100 mg／L 和200 mg／L 处理后细胞存活率均明显降低，并呈浓度和时间依赖性。不同浓度PM2．5可升高胞内 ROS 水平及增加 LC3 II 蛋白表达，亦呈浓度和时间依赖性。结论：PM2．5通过诱发氧化应激及自噬对气道上皮起到损害作用，其中氧化应激促进细胞过度自噬的发生。%Objective :To study the effects of PM 2 .5 on oxidative stress and autophagy of tracheal epithelial cell .Methods :After RTE cells were treated by different concentrations of PM 2 .5 collected and prepared ,cell incre‐ment rate was determined by M TT colorimetric method ,whether free radical generation of intracellular reactive oxygen species (ROS) was detected with flow cytometry , whether microtubule associated protein light 1 chain 3 I protein 1 (LC31) and LC3II was expressed with Western Blot .Results :After treated by PM 2 .5 100 mg/L and 200 mg/L ,Cell survival rate were significantly decreased with concentration and time dependence ,PM 2 .5 can also in‐crease the intracellular ROS level and LC3 II protein expression with concentration and time dependence .Conclu‐sion :Airway epithelia cells may be damaged by PM 2 .5 through oxidative stress and autophagy in which course oxida‐tive stress could promote cell excessive autophagy .
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