目的:探讨早期糖尿病视网膜病变(DR)状态下,缺氧诱导因子1αSymbolaA@_(HIF-1αSymbolaA@_)对血管内皮细胞表面细胞间粘附分子-1(ICAM-1)表达的影响.方法:收集早期DR患者及年龄匹配的健康人外周血血清,用于体外培养恒河猴视网膜血管内皮细胞系细胞(RF/6A).分为糖尿病血清培养组(B组)、糖尿病+HIF-1αSymbolaA@_反义寡核苷酸组(ASODN)(C组)、糖尿病+HIF-1αSymbolaA@_ 正义寡核苷酸组(SODN)(D组)健康人血清培养细胞为正常对照组(A组).使用免疫组织化学和免疫酶联吸附法(ELISA)检测HIF-1αSymbolaA@_和ICAM-1的表达水平.结果:在蛋白总量一致的情况下,A组细胞上清中sICAM-1的浓度为(13.89±1.35)ng/ml,而B组的浓度为(25.26±1.97)ng/ml ,HIF-1αSymbolaA@_ ASODN 转染可以明显降低细胞ICAM-1的表达水平,其浓度为(16.59±1.11)ng/ml,HIF-1αSymbolaA@_ SODN 转染则不能影响细胞ICAM-1的表达.各组细胞之间ICAM-1表达水平比较有统计学差异(F=81.89,P=0.00),各组之间两两比较,B组和D组之间比较无统计学差异(P=0.98),其余各组之间两两比较有统计学差异(P均<0.05). 结论:在体外培养条件下, HIF-1αSymbolaA@_对糖尿病血清培养条件下的RF/6A细胞表达ICAM-1具有调节作用.%Objective: To investigate the mediation of HIF-1 αSymbolaA@_ to the expression of ICAM-1 by retinal vascular endothelial cell under early stage of diabetic retinopathy condition.Methods:The rhesus choroid-retina vascular endothelial cell line RF/6A were cultured in RPMI 1640 medium-10% human serum, which was collected from the subjects of early stage of diabetic retinopathy and age-matched healthy control.The cells were cultured in 4 groups as control group(group A),diabetic group (group B), HIF-1 αSymbolaA@_ anti-sense oligonucleotides (ASODN) group (group C) and HIF-1 αSymbolaA@_ sense oligonucleotides (SODN) group (group D).ICAM-1 levels in RF/6A supernatants were determined with ELISA kit.Results: Contrast to group A, the level of ICAM-1 significantly increased in group B (25.26±1.97) ng/ml vs (13.89±1.35)ng/ml(P=0.00).But after HIF-1αSymbolaA@_ bioactivity was blocked (group C), the effect on the level of ICAM-1 by diabetic serum(group B) was suppressed significantly (25.26±1.97) ng/ml in group B(16.59±1.11) ng/ml in group C vs (25.26±1.97)ng/ml in group B(P=0.00).Conclusion: In vitro, HIF-1αSymbolaA@_ could regulate the expression of ICAM-1 by RF/6A.HIF-1αSymbolaA@_ may served as a therapeutic target for the treatment and/or prevention of early diabetic retinopathy.
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