Objective:To investigate the function of Raf‐1 in cell proliferation and cell cycles in vascular endothelial cells .Methods :The Human Umbilical Vein Endothelial Cells(HUVECs) were transfected with the eu‐karyotic expression vector pCDNA3‐raf‐1 or Small interference RNA Raf‐1‐siRNA .Then ,the cells growth curves were draw according of MTT ,and cell cycles were detected by FACs .Results:After the HUVECs were transfected with the eukaryotic expression vector pCDNA3‐raf‐1 ,the proliferation of HUVECs increased significantly than the control .Vice versa ,after the HUVECs were transfected with Raf‐1‐siRNA ,the Raf‐1 expression decreased ,and the amplification of HUVECs was slower down than the control ,the cell cycles were arrested at G1 phase .Conclu‐sion:The raf‐1 plays an important role in the growth of vascular endothelial cells .%目的：探讨Raf‐1蛋白激酶在血管内皮细胞生长增殖中的作用。方法：分别应用Raf‐1蛋白激酶的真核表达载体pCDNA3‐raf‐1及干涉核苷酸片段Raf‐1‐siRNA转染人脐静脉内皮细胞（HUVECs），进而应用MTT法绘制HUVECs生长曲线，应用流式细胞仪分析细胞周期。结果：HUVECs转染真核表达载体后，细胞内Raf‐1明显增高，与对照组比较，细胞增殖速度明显加快；而转染Raf‐1‐siRNA后，HUVECs细胞中Raf‐1表达水平降低，与对照组比较，细胞增殖速度明显减慢，阻滞于G1期。结论：Raf‐1蛋白激酶在血管内皮细胞生长增殖中发挥重要作用。
展开▼
