Objective:To observe the changes in the expression of silent information regulator 3 (SIRT3 ) in the cerebral motor cortex of rats following global cerebral ischemic reperfusion .Methods:The cerebral tissues were collected from rats following global cerebral ischemic reperfusion at time point of 6h ,24h ,72h ,5d ,and 7d (7 in each group) ,and the expression of SIRT3 in the cerebral cortex was detected by immunohistochemistry .Results :Under optical microscope ,SIRT3-positive cells were found in every group .Within the time range of 6h ,24h and 72h following global cerebral ischemic reperfusion ,SIRT3-positive cells increased significantly (P<0 .01) ,but within the time range of 72h ,5d and 7d ,the number of SIRT3-positive cells reduced significantly (P<0 .01) .Conclusion :The parabola-like change of SIRT3 expression time-phase in the cerebral cortex indicate that SIRT 3 might be related to pathophysiological processes of cerebral ischemic reperfusion .%目的：探讨全脑缺血再灌注大鼠大脑运动皮质内沉默信息调节因子3（SIRT3）的表达规律。方法：应用免疫组织化学染色方法测定全脑缺血再灌注后不同时间点（6h、24h、72h、5d及7d组）大鼠大脑运动皮质内SIRT3的表达情况。结果：6h组、24h组至72h组脑缺血再灌注大鼠大脑运动皮质内SIRT3的含量随再灌注时间延长而增加（ P＜0．01）；72h组、5d组至7d组大鼠大脑运动皮质内SIRT3含量随再灌注时间延长而减少（P＜0．01）。结论：全脑缺血再灌注后大鼠大脑运动皮质内SIRT3的表达随再灌注时间延长呈抛物线改变，提示SIRT3可能参与脑缺血再灌注损伤的病理生理过程。
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