目的：探讨透明质酸钠对术后腹膜粘连的预防作用。方法：将60只大鼠随即分为A组（创伤组）、B组（创伤＋透明质酸钠组）、C组（对照组），建立动物模型，分别于术后第1、3、5、7d处死动物，观察粘连情况及程度，用免疫组化SABC法检测TGF-β1表达情况。结果：①除C组外术后均形成腹腔粘连，其中A 组术后1、3d粘连评分无明显差异，3、5、7d间粘连评分有显著性差异；②B组术后1、3、5、7d粘连分级无显著性差异，B组5、7d粘连评分明显低于A组同时间点粘连评分；③B组TGF-β1的表达低于A组，A、B组TGF-β1的表达明显高于对照组。结论：腹膜粘连形成的关键时期是术后3～7d ；透明质酸钠可减少术后腹膜粘连的程度，能够抑制损伤组织愈合过程中TGF-β1的过量合成及表达。%Objective :To investigate of sodium hyaluronate on the prevention of postoperative perito-neal adhesions .Methods:60 rats were randomly divided into group A (trauma group) ,group B (trauma+ sodium hyaluronate group) ,group C (control group) ,the establishment of animal models ,respectively ,on postoperative day 1 ,3 ,5 ,7 the animals were sacrificed .To observe adhesions and extent of the TGF-β1 expression detected by immunohistochemistry SABC method .Results :① In addition to the group C ,postoperative formation of abdominal adhesions ,the adhesion score of group A after 1 ,3 days no significant difference 3 ,5 ,7 days a significant difference between the adhesion score ;②1 ,3 ,5 ,7 days in group B ,adhesion grading was no significant difference ,Group B , 5 ,7-day adhesion scores were significantly lower adhesion score of Group A at the same time point .③ TGF-β1 ex-pression in group B lower in group A ,A and B of TGF-β1 expression was significantly higher than control group . Conclusions :The critical period of peritoneal adhesion formation is 3-7 days after the operation ;Sodium hyaluronate can be reduced to the extent of postoperative peritoneal adhesions and can inhibit the healing process of damaged tis-sue excess of TGF-β1 synthesis and expression .
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