目的:探讨不同稀释碘伏浓度对人结肠癌细胞SW480增殖的影响.方法:用不同浓度的稀释碘伏(0.05%、0.025%、0.01%)作用于人结肠癌细胞株SW480,用MTT检测碘伏对结肠癌细胞增殖的影响,碘化丙啶(PI)染色观察稀释碘伏对肿瘤细胞形态学的改变.结果:不同浓度的稀释碘伏都抑制结肠癌细胞的增殖,抑制率可达到84.5%,浓度和时间依赖关系不明显.碘化丙啶染色后观察到细胞膜皱缩、胞核固缩、裂解等形态学改变.结论:稀释碘伏抑制体外培养结肠癌细胞的增殖,为稀释碘伏预防肿瘤在腹腔的种植转移提供理论基础.%Objective:To study the effects of diluted povidone-iodine on the proliferation activity of colon cancer cells SW480 at different doses. Methods:The cultured colon cancer cells SW480 were treated with different dose povidone-iodine (0. 05 % ,0. 025% ,0. 01 %). MTT assay was used to detect the proliferation activity. PI stai-ning was used to observe the morpnological changes of colon cancer cells. Results: Diluted povidone-iodine could sig-nificantly inhibit the colon cancer cells proliferation activity at different doses, but no obvious dependence on concen-tration and time was observed. After PI treatment, cell shrinkage, nucleus condensation, and chromosome breaking could been seen. Conclusion: Diluted povidone iodine can inhibit the proliferation of human colon cancer cell line SW480 in vitro, which would provide a theoretical basis for using diluted povidone iodine in intraoperative peritoneal lavage to inhibit the tumor metastasis.
展开▼
