目的:探讨氟比洛芬酯用于超前镇痛是否影响血小板的活化状态,为其安全用于围术期疼痛治疗提供理论依据.方法:选择Wistar大鼠24只随机分为4组,每组6只.Ⅰ组(生理盐水对照组)大鼠右后脚趾部皮下注射5％福尔马林溶液0.1 ml之前10 min腹腔注射生理盐水溶液2ml.Ⅱ组(即刻镇痛组)大鼠右后脚趾部皮下注射5％福尔马林溶液0.1 ml即刻腹腔注射氟比洛芬酯50mg/kg溶液2ml.Ⅲ、Ⅳ组(超前镇痛低、高剂量组)致痛之前10min腹腔注射25、50mg/kg氟比洛芬酯2ml.致痛后立刻进行疼痛评分；并且分别于致痛前24h、后12h取静脉血检测血小板CD62p水平.结果:预注氟比洛芬酯后有效抑制了相痛反应(P＜0.05),对Ⅰ相无影响(P＞0.05).与Ⅰ组比较,致病后12h血小板CD62p水平、Ⅲ、Ⅳ组均降低(P＜0.05),且Ⅳ组低于Ⅲ组(P＜0.05).结论:氟比洛芬酯可预防血栓栓塞性疾病.%Objective :To observe the Preemptive analgesic effect of flurbiprofen axetil on the CD62p level of platelet in the inflammatory painful model formalin-induced rats . Methods: Rats were randomized to either I of IV groups (n = 6 rats for each group): Group I received a intraperitoneal injection of 2 ml of normal saline solution 10min prior to intraplantar injection of 0. 1 ml of 5% formalin solution; Group Ⅱ received a intraperitoneal injection of 2 ml of 50mg/kg flurbiprofen axetil while intraplantar injection of 0. 1 ml of 5% formalin solution,Group Ⅲ 、 IV received a intraperitoneal injection of 25、50mg/kg 2 ml of flurbiprofen axetil respectively prior to intraplantar injection of 0. 1 ml of 5 % formalin solution. According to Pain reaction weighting score of Dubbisson and Dennis, the accumulative pain score of 0～5min (I phase) and 15～30 min (phase) after formalin injection were recorded respectively. The levels of platelet CD62p were measured respectively. Results : Intraperitoneal injection of flurbiprofen axetil prior to intraplantar injection of 0. 1 ml of 5% formalin solution inhibit the phase reaction of formalin-induced rats dependently (P <0. 05),but not had significant influence on the I phase(P >0. 05). Compared to group I , the level of platelet CD62p were significantly less in those of group Ⅲ 、Ⅳ , while that of group Ⅳ were less than group Ⅲ significantly (P< 0. 05) . Conclusion : flurbiprofen axetil can prevent the thrombotic diseases through interfering in activation of platelet.
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