目的:研究抑癌候选基因NDRG2在体外对人脑胶质瘤细胞系U251增殖和凋亡的影响.方法:将人脑胶质瘤细胞系U251细胞分成空白对照组,重组人ndrg2腺病毒组和今又生(重组人P53腺病毒)组,重组人ndrg2腺病毒组和今又生组下设1.6 8×1011、8.4×1010、4.2×1010 、2.1×1010、1.05×1010、5.25×109、5.25×108 、5.2 5×107 v.p/ml组,各组分别作用96 h后观察细胞形态学改变,甲基噻唑基四唑法(MTT法)绘制细胞生长抑制曲线.结果:重组人ndrg2腺病毒和今又生转染人胶质瘤细胞系U251后,细胞生长受到明显抑制,呈现明显的剂量-效应依赖曲线；重组人ndrg2腺病毒组对U251细胞的抑制率优于今又生组.结论:过表达NDRG2可明显抑制人胶质瘤细胞U251的增殖并导致细胞凋亡.%Objectiv, To investigate the function of candidate tumor suppressor gene ndrgZ on proliferation and apoptosis of human neuroblastoraa cell line U251 cells. Methods: U251 cells be treated with 8 concentrations of Recombinant Human Ad-ndr?2 and Recorobinant Human Ad-p53 (1.68× 10" .8.4 × 10* .4. 2×10IB 2.1×10" . 1,05X10'° .5.25X10* ,5.25X lo1,5.25X10' v. p/ml) for 96 h respectively. The morphological change* of the cells were observed under light microscopes. Cell viability was accessed by using colorimetric MTT assay. Resultst As compared with normal U251 cells* the growth was markedly slowed down in ndrg2 over expressing cell* and in PS3 over expressing cells. Recotnbinant Human Ad-ndrg2 inhibited the viability of UZ51 cells in a dose-and effect-dependent manner. There were significant difference between the Recombinant Human Ad-ndrg2 group and the Recotnbinant Human Ad-p53 group respectively . Conclusion ( ndrg2 gen* can significantly inhibit cell proliferation and markedly induce the apoptosts of U2S1 cells in vitro.
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