Objective : To investigate the effect of N-acetylcysteine on islet cell cultrued with palmitic acid.Methods:The cultrued islet cells (INS-1)were divided into 5 groups : normal control group, palmitic acid (PA) group(0.25mmol/L), 50μmol/L NAC+ PA group, 100μmol/L NAC+ PA group, 200μmol/L NAC+PA group.The level of insulin, GSH-Px and MDA in Culture supernatant were measured when cultrued for 24h.Result:Palmitic acid had the effects of inhibiting insulin secretion of pancreatic islet cell, reducing the concentration of GSH-Px and enhancing the level of MDA.This change were inhibited by NAC with a dosedependent manner.Conclusion : NAC protected islet cell against injury induced by palmitic acid through inhibiting oxidation in dose-dependent manner.%目的:探讨N-乙酰半胱氨酸(NAC)对软脂酸培养下胰岛细胞的影响.方法:体外培养胰岛细胞INS-1,分为空白对照组,软脂酸组(0.25 mmol/L),NAC作用组(浓度分别为50μmol/L,100μmol/L,200μmol/L),培养24h后,取上清测定胰岛素水平及GSH-Px、MDA含量.结果:软脂酸可使胰岛素分泌量减少,GSH-Px浓度下降,MDA含量增加,NAC可以明显抑制软脂酸引起的上述变化,抑制作用随NAC浓度的增加而增强.结论:抗氧化剂NAC对软脂酸导致的胰岛细胞损伤有一定的保护作用,并且呈浓度依赖性.
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