标准曲线的斜率应为0.081 ± 0.003吸光度/μg氨,但在实际实验中结果经常会偏离这个数值.由于配制标准工作液(将标准贮备液用吸收液稀释1000倍)时,如果使用100mL容量瓶,需量取贮备液0.1mL,由于量取贮备液的量很少,配制过程中稍有误差就会对曲线的斜率造成很大影响,所以配制标准工作液所使用容量瓶的大小对标准曲线的斜率影响不容小觑.%The slope of the standard curve should be 0.081 +0.003 absorbance/μg ammonia,but in practical experiments,the results often deviate from this value. Due to configuration standard working fluid(standard reserve with absorption liquid will be diluted 1000 times),if use 100 mL volumetric flask,take 0.1 mL reservior fluid demand,by measuring the amount of reserve liquid is small,a slight error in the configuration process will be great effects on the curve of the slope,so the size of the configuration standard working fluid by using volumetric flask effect on the slope of standard curve to be reckoned with.
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