The fluorescence quenching system between quercetin and rhodamine 6G was studied by the Stern- Volmer equation and the UV- Vis absorption spectrum. The static fluorescence quenching process occurred between them. Their binding constants and the numbers of binding sites were obtained with k1 = 2.63 x 10^6L .mol^-1, n1 = 1. 14 and k2 = 7.42 x 10^5L . mol^-1, n2 = 1. 07. Two linear relationships were got between F0/F and the concentration of quercetin in the ranges of 0.01 ~ 0.21ug . mL^-1 and 0.2 -5.0ug . mL^-1 respectively with the detection limit of 5.9ng . mL^-1 and the recovery of 91.7% - 104%. The method can be used for quantitative determination of quercetin.%以Stem-Volmer方程和罗丹明6G吸收光谱研究了槲皮素-罗丹明6G荧光猝灭体系。结果表明，槲皮素、罗丹明6G间发生静态猝灭，生成稳定复合物，二者的结合常数和结合点数为k1=2．63×10^6.mo^1，n1=1．14；k2=7．42×10^5·mol^-1，n2=1．07。槲皮素的质量浓度在0．01～0．2ug·mL^-1和0．2-5．0ug·mL-1范围内与F0／F成正比，检出限为5．9ng·mL^-1，加标回收率为91．7％～104％，方法可用于槲皮素含量测定。
展开▼
