Objective To observe effects of oridonin on killing,proliferation,and expression of NF-κB protein of hu-man glioma U251 cell line. Methods U251 cells were incubated with oridonin at the concentrations of 10,20,40,and 80 μmol/ L respectively. At the same time,U251 cells incubated without oridonin were taken as the control group. U251 counting,IC 50 ,U251 cell proliferation inhibition rate and expression of NF-κB were detected at different time points. Re-sults Compared with the control group,the number of U251 cells in the 40 and 80 μmol/ L oridonin groups decreased sig-nificantly (P < 0. 05). After incubation for 24,48 and 72 h,the IC 50 of U251 cells was 82. 2,65. 3 and 44. 1 μmol/ L, respectively. Compared with 20 μmol/ L oridonin group,the cell proliferation inhibition rates of the 40 and 80 μmol/ L ori-donin groups were significantly reduced at 48 and 72 h (P < 0. 05). Compared with the control group,the expression of NF-κB in U251 cells decreased significantly (P < 0. 05)in the 40 and 80 μmol/ L oridonin group at 48 and 72 h. Conclu-sions Oridonin can kill U251 cells,inhibit its proliferation and down-regulate NF-κB protein in vitro. The killing effect and inhibition can be related to the regulation of NF-κB signaling pathway.%目的 观察冬凌草甲素对人脑胶质瘤细胞株U251杀伤、增殖及NF-κB蛋白表达的影响.方法 将浓度为10、20、40、80μmol/L冬凌草甲素与U251细胞(实验组)分别孵育,同时设不加冬凌草甲素培养的U251细胞作对照组,分别于不同时间点计数U251、计算冬凌草甲素对U251细胞的IC 50、测算U251细胞增殖抑制率、检测U251细胞中的NF-κB蛋白.结果 与对照组相比,实验组加入40、80μmol/L的冬凌草甲素后U251细胞数明显减少(P均<0.05).冬凌草甲素作用24、48、72 h时对U251细胞的IC 50分别为82.2、65.3、44.1μmol/L.与20μmol/L冬凌草甲素相比,40、80μmol/L冬凌草甲素作用48、72 h时的细胞增殖抑制率明显降低(P均<0.05).与对照组相比,40、80μmol/L的冬凌草甲素作用48、72 h时,U251细胞中NF-κB蛋白的相对表达量明显降低(P均<0.05).结论 冬凌草甲素在体外可杀伤人脑胶质瘤细胞株U251,抑制其增殖,下调其NF-κB蛋白的表达;冬凌草甲素对U251细胞的杀伤、增殖抑制作用可与其对N F-κB信号通路发挥调节作用有关.
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