Objective To observe the changes in the proliferation and invasive abilities of human chorionic epithelio -ma cells after silencing Notch1 gene.Methods We looked up Notch1 sequence (NM 001105721.1) design primers pro-vided by Genbank to synthesize Notch 1 siRNA.Human chorionic epithelioma cells JEG-3 were cultivated and divided into groups A, B and C.The group B was transfected by control siRNA , group C was transfected by Notch1 siRNA and group A without any transfection.12, 24, 36,48, 60 hours after transfection, the cell proliferation ability was determined by MTT in each group , and the invasion ability was detected by Transwell invasive experiment .The expression of MMP-2 and MMP-9 protein was detected by Western blotting 72 h after transfection .Results The cell proliferation ability of group C at 36 h, 48 h and 60 h after transfection was lower than those of groups A and B (all P<0.05).The number of cells penetrating the membrane in the groups A , B and C were 109 ±6 , 118 ±7 and 59 ±4;significant difference was found between group C and groups A and B (all P<0.05).The relative expression of MMP-2 of groups A, B and C was 0.91 ±0.08, 0.87 ± 0.01 and 0.29 ±0.05, respectively.The relative expression of MMP-9 of groups A, B and C was 0.85 ±0.04, 0.79 ± 0.03 and 0.35 ±0.05, respectively.The expression of MMP-2 and MMP-9 in the group C was lower than that of groups A and B (all P<0.05).Conclusion The cell proliferation of human chorionic epithelioma is inhibited and the invasive a-bility is decreased after silencing Notch 1 gene.%目的：观察Notch1基因沉默后人绒毛膜上皮癌细胞增殖及侵袭能力变化。方法查阅GenBank提供的Notch1序列（NM-001105721．1）设计引物，合成Notch1 siRNA。分离培养人绒毛膜上皮癌JEG-3细胞，分为A、B、C组。 A组不做转染，B组转染control siRNA ，C组转染Notch1 siRNA。分别于转染后12、24、36、48、60 h采用MTT法检测各组细胞增殖能力（ OD值）。转染后48 h采用Transwell 侵袭实验检测细胞侵袭能力。转染后72 h采用Western blotting法检测各组细胞中的MMP-2、MMP-9蛋白。结果转染后36、48、60 h C组OD值低于A、B组（ P均＜0．05）。 A、B、C组穿膜细胞数分别为（109±6）、（118±7）、（59±4）个，C组与A、B相比，P均＜0．05。 A、B、C组细胞中MMP-2蛋白相对表达量分别为0．91±0．08、0．87±0．01、0．29±0．05，MMP-9蛋白相对表达量分别为0．85±0．04、0．79±0．03、0．35±0．05，C组MMP-2、MMP-9蛋白相对表达量与A、B组相比，P均＜0．05。结论Notch1基因沉默后，人绒毛膜上皮癌细胞增殖受到抑制，侵袭能力减弱。
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