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首页> 外文会议>International Conference on Mechatronics, Computer and Education Informationization >Research on the Effect of BMI1 Gene Silencing on the Proliferation Activity of Human Breast Cancer Cells
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Research on the Effect of BMI1 Gene Silencing on the Proliferation Activity of Human Breast Cancer Cells

机译：BMI1基因沉默对人乳腺癌细胞增殖活性的影响研究

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Objectives: The purpose is to study the effect of proto oncogene BMI1(B-cell specific moloney murine leukemiavirus insertion site 1) gene silencing on the proliferation inhibition and 5-Fu sensitivity of human breast cancer cells through experiment in vitro, and to explore the feasibility of the combined treatment of breast cancer by BMI1 gene silencing and 5-Fu. Methods: The MTT method was used to detect the inhibitory effects of 5-Fu on the proliferation of MCF-7 cells, MCF-7/5-Fu cells and their BMI1 gene silencing cells. Morphological changes of cells in each group stained with acridine orange were observed by fluorescence. The flow cytometry was used to detect the effect of 5-Fu on the apoptosis rate and cell cycle of each group. Results: 5-Fu inhibited the growth of human breast cancer cells, and presented time - dose dependence. At 48h, the IC50 of cells in each group inhibited by 5-Fu was respectively: MCF-7(2.85±0.21), MCF-7/BMIlsi (1.78±0.25), MCF-7/5-Fu(8.05±0.38) and MCF-7/5-Fu/BMI1si (5.65±0.27) μg/mL. BMI1 gene silencing cells were significantly lower than breast cancer cells(LSD-t=2.845, 3.232; P<0.05). If 4μg/mL 5-Fu acts on the cells of each group for 48h, it can induce obvious cell apoptosis features in MCF-7/5-Fu/BMI1si cells. The apoptosis rate of BMI1 gene silencing group was significantly higher than that of breast cancer cells MCF-7 and MCF-7/5-Fu(LSD-t=7.826, 8.231; P<0.05). The cell proportion of BMI1 gene silencing cells in G0/G1 phase also increased significantly (LSD-t=10.512, 8.053; P<0.05). Conclusions: BMI1 gene silencing can enhance the sensitivity of breast cancer cells to 5-Fu and promote cell apoptosis.
机译：目的是研究Propo癌基因BMI1（B细胞特异性Moloney鼠白血病插入位点1）基因沉默于体外实验对人乳腺癌细胞增殖抑制和5-FU敏感性的影响，并探索BMI1基因沉默和5-FU的乳腺癌结合治疗的可行性。方法：使用MTT方法检测5-FU对MCF-7细胞增殖，MCF-7/5-FU细胞及其BMI1基因沉默细胞的抑制作用。通过荧光观察用吖啶橙染色的每组细胞的形态变化。流式细胞术用于检测5-FU对每组凋亡率和细胞周期的影响。结果：5-FU抑制人乳腺癌细胞的生长，并呈现时间 - 剂量依赖。在48小时，每组中的细胞IC50分别抑制5-FU：MCF-7（2.85±0.21），MCF-7 / Bmilsi（1.78±0.25），MCF-7/5-FU（8.05±0.38）和MCF-7/5-FU / BMI1SI（5.65±0.27）μg/ ml。 BMI1基因沉默细胞显着低于乳腺癌细胞（LSD-T = 2.845,3.232; P <0.05）。如果4μg/ ml 5-fu在每组的细胞上作用48小时，则可以在MCF-7/5-FU / BMI1SI细胞中诱导明显的细胞凋亡特征。 BMI1基因沉默组的凋亡率明显高于乳腺癌细胞MCF-7和MCF-7/5-FU（LSD-T = 7.826,8.231; P <0.05）。 G0 / G1相中BMI1基因沉默细胞的细胞比例显着增加（LSD-T = 10.512,8.053; P <0.05）。结论：BMI1基因沉默可以增强乳腺癌细胞对5-FU的敏感性，促进细胞凋亡。

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