Objective To establish rat ischemia reperfusion injury model and evaluate the protection effect of querce -tin against liver ischemia reperfusion injury in rats .Methods A total of 80 healthy rats were randomly divided into control group(n=5), sham group(n=25), ischemia reperfusion injury group(n=25) and quercetin group(n=25).The later three groups were divided into 0, 6, 12, 24, 72 h subgroups(n=5), respectively.At the end of the experiment, the blood samples and liver tissues were collected .The levels of ALT, AST, IL-1β, IL-6, IL-8 and TNF-αwere determined by automatic biochemistry analyzer and competitive inhibition ELISA , respectively .The morphology change of liver were de-tected by HE staining, and the apoptosis of liver cells were detected by TUNEL .Results In 0, 6, 12, 24, 72 h sub-group, the levels of ALT, AST, IL-1β, IL-6, IL-8 and TNF-αincreased, especially in 12 h group, which had significant statistically difference compared with control or sham group (all P<0.05).Under light microscope, there was no signifi-cant change in control or sham group .In ischemia reperfusion injury group , liver cells degenerated significantly , liver cord structure disappeared, some liver cells necrosed.However, in quercetin group, only part liver cells degenerated , part liver cord structure exsited , which was consistent with the results of apoptosis index .Conclusion Quercetin is beneficial for liver ischemia reperfusion injury .%目的：评估槲皮素对缺血再灌注大鼠肝脏损伤大鼠的保护作用。方法健康大鼠80只被随机分为对照组5只、假手术组25只、缺血再灌注组25只和槲皮素组25只，后3组再按不同灌注时限（0、6、12、24、72 h）分为5个亚组，每个亚组5只。分别于实验结束后留取大鼠血液及肝脏组织，用全自动生化分析仪检测血清中 ALT、AST水平，竞争抑制ELISA法检测血清中IL-1β、IL-6、IL-8、TNF-α水平；HE染色法观察肝脏的形态学改变，并用原位末端标记法检测肝脏细胞的凋亡情况。结果肝脏缺血再灌注组各时点血清中ALT、AST、IL-1β、IL-6、IL-8、TNF-α均有所升高，且以12 h最高，与对照组、假手术组相比差异有统计学意义（P均＜0．05）；除IL-6外，槲皮素组各时点以上检测指标与缺血再灌注组比较有统计学差异（P均＜0．05）。光镜下，对照组、假手术组肝脏无明显改变；而在缺血再灌注后12 h，肝细胞变性明显，肝索结构消失，部分肝细胞灶状坏死；槲皮素组的肝脏仅部分细胞变性，部分肝索结构存在，该形态学变化与细胞凋亡指数的检测结果一致。结论槲皮素能够有效地改善大鼠肝脏缺血再灌注损伤。
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