目的 探讨Notch1和Jagged1蛋白在大肠腺癌发生发展的作用.方法 采用免疫组织化学PV -9000法和Western blotting法检测78份大肠腺癌组织(大肠癌组),40份大肠腺瘤组织(腺瘤组)及40份正常大肠组织(距大肠腺癌组织远端5 cm,正常组)Notch1与Jagged1蛋白表达情况,分析其与大肠癌临床病理参数的关系.结果 大肠癌组Notch1、Jagged1蛋白阳性率明显高于腺瘤组及正常组,P＜0.05；Notch1和Jagged1蛋白表达强度与大肠癌组织学分级、Dukes分期和淋巴结转移密切相关,P均＜0.05.大肠癌组Notch1与Jagged1蛋白表达关系呈正相关(r =0.407,P＜0.01).结论 Notch1和Jagged1蛋白在大肠腺癌的发生与发展中起重要作用.%Objective To investigate the roles of Notchl and Jaggedl protein in the occurrence and development of colorectal adenocarcinoma(CA). Methods Immunohistochemical PV-9000 method and Western blot were used to detect the expression of Notchl protein and Jaggedl protein in 78 cases of CA( CA group ) , 40 cases of colorectal adenoma( adenoma group) and 40 cases of normal c lorectal tissue (5 cm far from the CA, normal group) , and their relationship with the clinic pathological parameters were analyzed. Results The positive expression rate of Notchl and Jaggedl protein in CA group were significantly higher than those in adenoma group and normal group (P < 0. 05 ) ;the expression intensity of Notchl and Jaggedl protein were closely associated with histological grading, Dukes stage and lymph nodemetastasis of CA ( P < 0. 05 ) . There was positive correlation between the expression of Notchl and Jaggedl protein in CA group ( r = 0. 407, P <0.05). Conclusion Notchl and Jaggedl protein play important roles in the pathway of carcinogenesis and progression of CA.
展开▼
