目的 探讨Notch1信号通路对体外培养的胃癌细胞(BGC-823)增殖的影响,为胃癌的治疗提供新思路.方法 将体外培养的胃癌细胞株BGC-823分为三组,空白组加入PBS缓冲液,激活剂组加入Notch1通路激活剂rh-NF-κB,抑制剂组加入Notch1通路抑制剂γ-secretase inhibitorⅡ.Western blot法检测各组Notch-1和Hes-1蛋白表达；MTT法检测细胞增殖情况；流式细胞仪检测细胞周期分布.结果 与空白组比较,激活剂组Notch-1和Hes-1呈高表达、抑制剂组呈低表达；激活剂组细胞增殖减低,抑制剂组增殖旺盛；激活剂组S期胃癌细胞所占比例低,抑制剂组所占比例高.P均＜0.05.结论 Notch1信号通路激活能抑制胃癌细胞增殖.%Objective To investigate the Influence of Notch 1 signaling pathway on the proliferation of gastric cancer cells ( BGC-823) cultured in vitro, so as to provide new ideas for the treatment of gastric cancer. Methods Gastric cancer cells BGC-823 cultured in vitro were divided into three groups, PBS buffer liquid was added to the first group as blank group, Notchl signaling pathway activator rhNF-KB was added to the second group as activator group, Notchl signaling pathway inhibitor γ-secretase inhibitor II was added to the third group as inhibitor group. Western Blot method was used to detect the expression of Notch-1 and Hes-1 protein; MTT method was used to detect the change of cell proliferation; Flow Cytometric Analsis was used to test the cell cycle distribution. Results Compared to the blank group, the expression of Notch-1 and Hes-1 protein in the activator group was significantly high, while which was opposite in the inhibitor group. Compared to the blank group, cell proliferation in the activator group was inhibited, while which was opposite in the inhibitor group; the cell cycle distributions of gastric cancer cells in the 3 group were different to each other, compared to the blank group, the percentage of gastric cancer cells in the S phase was significantly low in the activator group, while which was opposite in the inhibitor group (P <0.05). Conclusion The activation of Notchl signaling pathway can inhibit the proliferation of gastric cancer cells, the inhibition of Notchl signaling pathway can promote the proliferation of gastric cancer cells.
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