Objective To study the protective effects of memantine hydrochlonde on damaged optic nerve in rabbits. Methods Sixty-six healthy rabbits were randomly divided into 3 groups which were control group ( group A, 18 rabbits) , injured NS group (group B, 24 rabbits) and injury treatment group(group C, 24 rabbits). Group B and C were randomly divided into two groups of only injuries (group Bl , 18 rabbits) , injury treatment group(group B2, 6 rabbits) , group Cl (18 rabbits) , group C2 (6 rabbits). Rabbits in group B and C were made optic nerve injury model. Each rabit in group C received memantine hydrochloride drug treatment with daily dose of 10 mg and once daily morning to all drugs. Each rabit in group B received normal saline. Respectively, three rabbits in group A, group Bl and group Cl were randomly killed at 1, 3 , 7 , 14, 21, 28 day after optic nerve injuried to detect changes in retinal concentrations of glutamate. In group B2 and group C2, 1 rabbit was killed to detect the retina pathological changes. Results After optic nerve injury (group B and group C) glutamate concentration increased rapidly in rabbits retina, compared with the control group A, all P <0.05. Glutamate concentration was significantly lower in C group than group B(P<0.05). Conclusion Glutamate receptor antagonist memanline hydrochloride can reduce retinal glutamate concentration and optic nerve cell apoptosis.%目的 观察谷氨酸受体拮抗剂盐酸美金刚对兔受损视神经的保护作用.方法 健康大白兔66只,随机分为3组,对照组(A组)18只、损伤生理盐水组(B组)24只、损伤治疗组(C组)24只,B、C两组又随机分为B1组18只、B2组6只及C1组18只、C2组6只.B、C两组兔子双眼制作视神经损伤模型后,C组每日上午一次性给予盐酸美金刚灌胃,每日每只兔子10 mg;B组给予生理盐水5mL灌胃,给药时间同C组.造模后第1、3、7、14、21、28天随机处死A组兔子3只、B1组3只、C1组3只,检测其视网膜谷氨酸,同样时间点随机处死B2组兔子1只、C2组1只,观察视网膜病理形态学.结果 兔视神经损伤后(B、C组)视网膜谷氨酸浓度迅速增高,与A组相比,P均＜0.05.C组谷氨酸浓度明显低于B组(P＜0.05).结论 谷氨酸受体拮抗剂盐酸美金刚能明显降低视网膜谷氨酸浓度,减少视神经细胞凋亡.
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