目的 探讨GL50分子在活化T细胞表面的表达及其生物学意义.方法 分别取不同时相(24～144 h)激发型CD3单抗联合CD28单抗活化的T细胞,采用流式细胞术分析T细胞表面GL50分子表达阳性率,RT-PCR法检测T细胞内GL50分子mRNA转录水平；3H-TdR掺入法分析阻断GL50信号对T细胞增殖的影响.结果 激发型CD3单抗联合CD28单抗活化T细胞后24 h GL50分子阳性表达率为17.2％,其后逐渐升高至活化120 h时达最大阳性率95.7％,并维持至活化144h时基本不变；T细胞活化24～144 h均可检测到GL50分子mRNA表达；阻断性GL50分子单抗对活化T细胞增殖具有显著抑制作用.结论 GL50分子在活化T细胞表面呈诱导性表达,且可与T细胞表面自身受体结合在免疫应答放大效应中发挥重要作用.%Objective To explore the expression of GL50 on activated T cells and its biological significance. Methods Time courses of T cells activated by agonistic anti-CD3 plus anti-CD28 were chosen,then the positive rate of GL50 expression was analyzed through flow cytometry,the level of GL50 Mrna analyzed by RT-PCR. Effects of GL50 on T cells were measured by 3H-TdR incorporation. Results The positive percentage of GL50 on activated T cells at 24 h was 17.2% , and then rapidly increased to 95.7% at 120 h after activation,and which maintained stable to the 144 h; the expression of GLSO Mrna could be detected at 24-144 h after the activation of T cells; antagonist GL50 mAbs could inhibit the proliferation of T cells. Conclusion GL50 molecule can express inductively on activated T cells, and play an important role during the course of immune response through interaction of its corresponding receptor.
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