目的 比较肾上腺皮质癌(ACC)与其癌旁组织的蛋白表达谱的差异,筛选新的诊断ACC的肿瘤标志物.方法 分别提取12例ACC组织蛋白及其癌旁组织蛋白.蛋白定量后,采用双向凝胶电泳(2-DE)对组织蛋白表达谱进行分离,采用飞行时间质谱对差异位点进行蛋白鉴定.结果 共鉴定出29个ACC相关蛋白,其中表达上调20个,下调9个.上调蛋白的分子功能主要为蛋白质结合和氧化还原酶活性功能.结论 成功筛选出一组在ACC组织中表达差异的蛋白,可能为ACC的候选肿瘤标志物.%Objectives To compare the proteomic profiling of adrenal cortical carcinomas( ACC) and paratumorous tis sues, and identify novel tumor markers. Methods Protein samples from 12 ACC tissues and their paired paratumor tissues were extracted, and isolated by two-dimensional electrophoresis. Differently expressed protein spots were identified by mass spectrometry. Results 20 up-regulated dnd 9 down-regulated proteins in ACC tissues were identified compared with nor mal controls. Most of the up-regulated proteins were focused in protein binding and oxidoreductase activity in molecular function classification. Conclusion The differently identified proteins in this study are novel candidate biomarkers for ACC.
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