研究了用超临界CO2萃取法从白芷中提取欧前胡素和异欧前胡素，根据正交试验设计确定了超临界萃取最佳工艺条件为温度60℃、萃取压力25MPa和药材粉碎度40～60目，并放大50倍进行萃取试验。利用高速逆流色谱技术分离纯化了欧前胡素和异欧前胡素，溶剂系统为正己烷：乙酸乙酯：甲醇：水（I：0．4：1．1：0．5，V／V）。采用HPLC．DAD，MS对欧前胡素和异欧前胡素进行了定性定量分析，得到产物纯度达99．0％以上，本方法简便快捷，重现性好。%This paper addressed the extraction of imperatorin and isoimperatorin from Angelica dahurica （Fisch. ex Hoffm. ） with supercritJcal CO2 extraction method. Orthogonal experiments show that the optimal technological conditions of this extraction method are temperature of 60 ℃, pressure of 2.5 MPa, sample particle size of 40 -60 mesh and 50 times amplification of a preparative supercritical fluid extraction （SFE） system. We separated and purified imperatorin and isoimperatorin with high-speed counter-current chromatography and a two-phase solvent system of 1 ： 0.4： 1. 1： 0.5 （ n- hexane-ethyl vs. acetate vs. methanol vs. water, V/V）. We also employed HPLC-DAD and MS to perform quantative and qualitive analysis for imperatorin and isoimperatorin. The purities of them were both above 99.0%. The method is convenient and fast,with a high reproducibility.
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