Objective To investigate the effects of perfluorocarbon (PFC ) before and after intratracheal LPS administration on acute lung injury .Methods Fifty-two male ICR mice were prepared .Thirty-two mice were randomly divided into 4 groups ( n =8) ,C group:normal saline (NS) group ,L group:LPS group ,P1 group:PFC at 30 min before LPS instillation ,P2 group:PFC at 30 min after LPS instillation .The animals were executed at 3 hours after LPS instillation .The lung wet/dry weight ratio ,lung permeability were examined .Another 20 mice were also divided into the same 4 groups ( n=5) .Matrix metalloproteinase-2 (MMP-2) ,TNF-α,IL-8 con-tent in lung tissue were examined by ELISA .Neutrophils in bronchoalveolar lavage fluid (BALF) were detected . Results PFC reduced the lung wet /dry weight ratio ,reduced the total number of white blood cells and neutro-phil number proportion in BALF ,inhibited MMP-2 release ,reduced the content of TNF-αand IL-8 in lung tissue . Using PFC before LPS instillation have more obvious effects on each index than after (P<0 .05) .Conclusion U-sing PFC before LPS instillation have better protective effects on ALI by reducing TNF-αin lung tissue .%目的：探讨气道滴入脂多糖前后全氟化碳（PFC）对小鼠急性肺损伤（ALI）的影响。方法雄性ICR小鼠52只，分为2批，第1批32只，第2批20只，每批分为4组，正常对照组：0.9％氯化钠注射液（NS）对照组；脂多糖（LPS）组：采用LPS气道滴入诱导小鼠ALI模型，P1组：LPS滴入30 min前用PFC ，P2组：LPS滴入30 min后用PFC。L PS气道滴入后3 h处死动物，第1批左肺灌洗，检测白细胞及中性粒细胞计数，右肺上叶石蜡切片苏木素-伊红（HE）染色，做病理分析，中下叶酶联免疫吸附试验（ELISA）法检测肺组织匀浆肿瘤坏死因子（TNF）-α、白细胞介素（IL）-8、肺组织基质金属蛋白酶2（MMP-2）含量；第2批处死前30 min尾静脉注射1％伊文斯蓝染料0.1 mL ，取左肺测定湿质量/干质量比、取右肺测肺通透性。结果 PFC降低肺湿质量/干质量比及肺通透性，降低肺泡灌洗液中白细胞总数和中性粒细胞数比例，抑制组织MMP-2的释放，降低肺组织匀浆 TNF-α、IL-8的含量，L PS滴入前比滴入后使用PFC对上述各指标的作用更明显（ P＜0.05）。结论气道滴入L PS前比滴入后用PFC更能减轻LPS诱导的ALI ，可能通过减少肺组织产生TNF-α减轻肺损伤。
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