二酪氨酸是酪氨酸氧化的标志性产物.为研究影响羟基自由基诱导酪氨酸氧化的因素,采用同步荧光光谱结合二维相关技术研究羟基自由基诱导酪氨酸氧化的反应过程.结果表明:pH变化时,二酪氨酸的荧光峰位、峰强发生变化.在羟基自由基诱导酪氨酸氧化体系中,随着酪氨酸浓度的增加,二酪氨酸产量升高;随着过氧化氧浓度的增加,二酪氨酸产量降低;酸性条件下,该反应易于发生,碱性条件下该反应难以发生;随着反应的进行,二酪氨酸荧光强度先增加后减小.二维同步荧光光谱相关分析表明,292 nm处荧光强度的变化快于281,300和374 nm处荧光强度的变化.因此,荧光光谱法研究羟基自由基诱导酪氨酸氧化,简单可行.%Dityrosine is a marker of tyrosine oxidation. To study effecting factors of hydroxyl radical on tyrosine oxidation,synchronous fluorescence spectra with two dimensional correlation was used. The results showed that the peak position and intensity of dityrosine changed while pH value varied. In the system of tyrosine oxidation, with the increment of tyrosine concentration, the concentration of dityrosine decreased. With the increment of hydrogen peroxide concentration, the concentration of dityrosine increased. The oxidation reaction was prone to taking place in acid conditions while difficult to develop in basic conditions. With the development of oxidation reaction, the fluorescence intensity of dityrosine increased and then decreased.Two dimentional correlation synchronous fluorescence spectra showed that the variation in the intensity at 292 nm preceded that of 281 , 300 and 374 nm. Thus, fluorescence spectroscopy was simple and easy for studying tyrosine oxidation induced by hydroxyl radical.
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