Objective To investigate the effect of the content of the bone marrow stromal cells in the bone marrow stro-mal cells induced by high glucose in rats under high concentration of glucose. Methods Selected June 2015 - 2016 in April in Heilongjiang Province Hospital Laboratory of 8 rats, experimental groups (each gene of five hole complex par-allel experiment): normal group (2% FBS DMEM culture cells), high glucose group (2% fetal bovine serum + glucose of both cultured cells), icariin group 1 (2%fetal bovine serum DMEM+icariin cultured cells) and icariin group 2 (2%fe-tal bovine serum and high glucose increased + icariin cultured cells), every five holes in parallel experiments. The ex-pression of BMP2, OPN and BSP genes in the bone related genes was determined by fluorescence quantitative. Results Compared with other groups, the expression of BMP2 (1.37 ±0.04), OPN (1.38 ±0.09)and BSP (1.50 ±0.05)genes in the group of 1(1.78 ±0.10) and other groups was higher than that in the control group(P<0.05). Conclusion The effect of the content of the bone marrow stromal cells in the bone marrow stromal cells of rats under high glucose condition was enhanced by the high concentration of glucose in the rat bone marrow stromal cells.%目的：探讨淫羊藿苷对高糖培养条件下大鼠颌骨骨髓基质细胞成骨能力的影响。方法选取2015年6月—2016年4月黑龙江省医院实验室的8只大鼠，实验分组(每个基因进行5个复孔平行实验)∶正常组(2%胎牛血清+DMEM培养的细胞)，高糖组(2%胎牛血清+高糖-DMEM培养的细胞)，淫羊藿苷组1(2%胎牛血清+DMEM+淫羊藿苷培养的细胞)，淫羊藿苷组2(2%胎牛血清+高糖-DMEM +淫羊藿苷培养的细胞)，每组进行5个复孔平行实验。荧光定量测定成骨相关基因BMP2、OPN、BSP基因的表达。结果淫羊藿苷组1(1.78±0.10)与其它组比较∶BMP2(1.37±0.04)、OPN(1.38±0.09)、 BSP(1.50±0.05)基因的表达均高于其相应的对照组(P<0.05)。结论淫羊藿苷对高糖培养条件下大鼠颌骨骨髓基质细胞成骨能力有加强作用。
展开▼
