Thispaperattemptbytheful biodegradationofCrudeoilforproducemethanegas,canbedifficulttocontaintheremainingoilintoareservoir more easily evelopment gas reservoir. adding only one gram crude oil.In this study, bacteria resource was oil wel produced water at different blocks of DaqingOilfield,thesolemicrobialcarbonsourcewascrudeoil.Methanewasdetectedafter2monthsofcultureataclosedmediumunder45℃.Bymi-crobial metabolism, 81mL cumulative gas was obtained which was converted by 5g oil in 12 months.The flora can be divided into three categories ac-cording to the metabolic function, which include hydrocarbon degradation bacteria, acetic acid bacteria and methane- producing bacteria, and these threecategoriescompletedtheprocessofmethane- producingbycrudeoil.AndthescreenedMethanogenicArchaeawereidentifiedasMethanocul eus andMethanosaetathermophila.%本文通过对原油的充分降解生成甲烷气体，可以将不易开采含有剩余油的油藏变为更容易开采的气藏。以大庆油田不同区块油井产出水为菌种来源，利用原油为微生物唯一碳源。45℃封闭条件下培养。培养2个月后，开始检测到甲烷气体，5克原油底物通过微生物作用，12个月累计产气量为81mL。根据菌群代谢功能，可以分为三类，分别为烃降解菌、乙酸盐菌和产甲烷菌，这三大类菌完成了由原油生成甲烷的过程。对筛选出的产甲烷古菌进行分类鉴定，鉴定结果分别为甲烷囊菌属（Methanocul eus）和嗜热甲烷鬃菌（Methanosaeta ther-mophila）。
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