为了探明不同烤烟品种根系丛枝菌根真菌（AMF）多样性及群落结构的差异，以云南主栽烤烟品种云烟202、云烟87、云烟85和K326为材料，构建每个品种根系AMF 18S rRNA基因克隆文库。结果表明，在4个烤烟品种的文库中共发现了21个操作分类单元（OTU），云烟202、云烟87、云烟85和K326克隆文库的OTU数分别为6、9、8和8；OTU在烟草根系中的分布显示，OTU1和OTU2是4个烟草品种根系的主要类型，OTU3为常见类型，OTU4只在云烟85和K326中有分布，而其余类型仅在一个烟草品种根系中出现；系统发育分析表明，9个OTU类型可划分到属，其中球囊霉属（Glomus）5种，无梗囊霉属（Acaulospora）2种，硬囊霉属（Sclerocystis）2种，其余12个OTU类型具体分类地位不明确，可能为新的AMF种类。多样性分析结果表明，云烟87根系AMF多样性最为丰富，云烟85和K326品种间差异不明显，而云烟202中物种类型则最少。可见，侵染4个烟草品种根系的主要AMF类型相同，但稀有AMF类型则明显不同。%In order to investigate the diversity and community structure difference of arbuscular mycorrhizal fungi(AMF)in roots of four flue-cured tobacco varieties(Yunyan 202, Yunyan 87, Yunyan 85 and K326), the 18S rRNA gene clone libraries of AMF were constructed. The results showed that there were 21 operational taxonomic units (OTUs) in the four clone libraries. The number of OTUs in Yunyan 202, Yunyan 87, Yunyan 85 and K326 clone libraries were 6, 9, 8 and 8, respectively. According to the distribution of OUTs in the four tobacco varieties, OTU1 and OTU2 were the main types, with OTU3 being a common type and OTU4 only found in Yunyan 85 and K326. The other remaining gene types were only appeared in 1 of the four tobacco varieties. Phylogenetic analysis showed that 9 OTUs could be classified into genus: 5 of the OTUs belonging to Glomus, 2 of the OTUs belonging to Acaulospora and another 2 OTUs belonging to Sclerocystis. The taxonomic of the other 12 OTUs was not clear, they might belong to novel AMFs. Diversity analysis suggested that the AMF diversity of Yunyan 87 was the most abundant and there was no obvious difference between Yunyan 85 and K326. The type of AMF in Yunyan 202 was the least. In summary the main AMF types in the four tobacco varieties were the same, while rare AMF types were significantly different.
展开▼
