确定了一种简单,明确和灵敏的高效液相测定和检测超临界二氧化碳萃取物中生育酚的方法.通过加入携带剂,超临界二氧化碳从菜籽脱臭馏出物提取生育酚浓缩物,其分析在反向色谱柱Zorbax C18上,用98%甲醇作为流动相,UV检测波长为292nm,α-生育酚作标准物.此方法线性相关性较高.APCI-MS和APCI-MS2检测的各生育酚的m/e与理论预测值一致.%A simple, specific and sensitive high-performance liquid chromatographic method was developed for the determination and detection of tocopherols in extracts by supercritical carbon dioxide. A supercritical carbon dioxide extraction with cosolvents procedure was used to concentrate tocopherols from the rapeseed deodorize distillates. The analyses were separated on a Zorbax C18 reversed-phase column using 98% methanol as mobile phase, UV detection at 292 nm and α-tocopherol as a standard. The calibration graphs of the method were linear. Results by APCI-MS2 experiments were consistent with outcomes from theories.
展开▼
