为更好地实现好氧反硝化茵的脱氮性能,本研究基于前期筛选的一株好氧反硝化菌pseudomonas putida AD-1,从脱氮效率及产酶角度考察其不同CODCr/TN和DO下的反硝化脱氮性能.在一定CODCr/TN范围内,CODCr/TN与培养24h后的菌株脱氮效率及ORP的减少量均呈正相关.当CODCr/TN为20.7时,脱氮效率为59.68%;当CODCr/TN为2.1时,脱氮效率仅为7.53%.培养过程中NO2-N浓度虽始终处于较低水平却呈上下波动状态.AD-1菌的ODO在4.82 mg/L左右,与大多数的好氧反硝化菌相比具有更高的氧气忍受浓度.通过SDS-PAGE蛋白电泳可以明显看出不同DO下茵体与反硝化相关的蛋白表达差异性,为进一步研究好氧反硝化茵产酶特性奠定理论基础.%To achieve a better nitrogen removal performance of aerobic denitrifiers, the study investigated its denitrification activity under different CODcr/TN ratios and DO concentration at the angle of nitrogen removal rate and enzyme production based on an our previous screening an aerobic denitrifier Pseudomonas putida AD-1. Results indicated that, at a certain range of CODcr/TN ratios, the CODcr/TN ratios were positively correlated well with denitrification efficiency and ORP reduction after 24h cultivation. When CODcr/TN ratios were 20.7 and 2.1, denitrification efficiency were 59.68％and 7.53％, respectively. It was interesting that nitrite concentration was always at low level but fluctuated during the culturing process. The optimal DO concentration of AD-1 was approximate 4.82mg/L which demonstrated AD-1 could tolerate higher DO concentration than most aerobic denitrifiers. By SDS-PAGE difference analysis on the protein expression under different DO concentration laid a theoretical foundation for further study on enzymatic characteristic of aerobic denitrifiers.
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