Objective: This study aimed to investigate the role of Bax gene in apoptosis induced by hyperthermia in BcaCD885 cells. Methods: After inducing apoptosis through hyperthermia, the expression of Bax protein and mRNA were examined with FCM antibody analysis and RT-PCR technique respectively. Results: Up regulation of Bax protein was noted after hyperthermia. The correlation coefficient r between the apoptotic rate and the expression Bax protein was 0.9240. The expression of Bax mRNA was also raised after hyperthermia. Conclusion: Hyperthermia acting as an external signal may up-regulates expression of Bax gene and then induces apoptosis in BcaCD885 cells.%目的:探讨Bax基因在热诱导BcaCD885细胞凋亡过程中的作用。方法:水浴加温诱导BcaCD885细胞产生凋亡。通过FCM-DNA、FCM-抗体检测及RT-PCR技术,观测热诱导颊癌细胞凋亡过程中Bax基因蛋白及mRNA表达的动态变化。结果:凋亡率与Bax蛋白表达水平存在正相关关系;加热后,细胞Bax mRNA相对量明显升高。结论:高温作为一种外来刺激信号通过上调Bax基因的表达,诱导BcaCD885细胞凋亡的发生。
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