目的：了解徐州地区三级医院肺炎克雷伯菌产KPC酶流行状况。方法收集徐州地区三级医院临床分离非重复肺炎克雷伯菌130株，予VITEK-32型全自动微生物分析系统鉴定，筛选出对亚胺培南耐药或中介菌株，对初筛阳性菌予改良Hodge试验进行表型确认，PCR方法检测KPC基因。结果初筛41株肺炎克雷伯菌可疑产碳青霉烯酶，经改良Hodge试验测定35株KPC酶阳性，检出率为26.9%（35/130），PCR方法检测有33株KPC酶阳性，检出率为25.4%（33/130）。结论徐州地区三级医院产KPC酶肺炎克雷伯菌发生率相对较高，需引起临床关注。%Objective To understand the prevalence of KPC carbapenemase in Klebsiella pneumoniae in Xuzhou region. Methods A total of 130 nonrepeated isolates of Klebsiella pneumoniae from tertiary hospitals in Xuzhou region were collected, imipenem-resistant were screened by VITEK-32,then use Hodge testing and PCR methods to detect KPC carbapenemase . Rwsults Among 130 isolates strains 41 isolates were deteced to imipenem-resistant,35 strains were detected to produce KPC carbapenemase by Hodge testing (26.9%),33strains were detected to produce KPC carbapenemase by PCR methods (25.4%). Conclusion The incidence of producing KPC carbapenemase of K. pneumoniae strains in Xuzhou area is relatively high and the control should be strengthened.
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