目的：探讨鞣花酸对前列腺癌细胞PC3的增殖、迁移、趋化作用，以及对前列腺癌细胞PC3中IL-8信号通路的抑制作用。方法采用6μg/ml、12μg/ml浓度的鞣花酸分别处理前列腺癌细胞PC3，通过细胞计数实验观察PC3的细胞增殖、通过细胞划痕实验观察癌细胞的运动、通过Transwell趋化小室实验观察细胞趋化作用，同时观察6μg/ml、12μg/ml浓度的鞣花酸对前列腺癌细胞PC3中IL-8信号激活的抑制作用，探讨鞣花酸对前列腺癌细胞PC3的抑制作用机制。结果与对照组比较，经6μg/ml、12μg/ml浓度的鞣花酸对前列腺癌细胞PC3处理24h、48h、72h时，对抑制细胞增殖、迁移及趋化运动均有明显作用（P<0.05）；6μg/ml、12μg/ml浓度的鞣花酸能明显抑制IL-8对前列腺癌细胞AKT、ERK1/2的激活。结论鞣花酸可抑制前列腺癌PC3细胞增殖、迁移及趋化运动，并可抑制IL-8对前列腺癌细胞的信号激活作用，表明鞣花酸是重要的前列腺癌抑制剂，在预防前列腺癌复发及转移中可能有重要作用。%Objective To investigate the mechanism of ellagic acid on PC3 prostate cancer cells proliferation inhibition and Il-8 signal pathway inhibition effect.Methods Ellagic acid with 6μg/ml,12μg/ml different concentrations to treat prostate cancer cell PC3,ellagic acid on prostate cancer cells PC3 cell proliferation,cell movement,cell chemotaxis,and ellagic acid in prostate cancer cell PC3 IL - 8 signal activation were investigated,with the target to explore the ellagic acid inhibition mechanism of PC3 prostate cancer cells.Results Ellagic acid not only inhibits prostate cancer cell proliferation,its migrationand chemotactic movement,but also significantly inhibits IL - 8’s activation of prostate cancer cells,which indicates the ellagic acid as as inhibitor of prostate cancer with its important role inprevention of its recurrence and metastasis.Conclusion Ellagic acid can inhibit PC3 prostate cancer cell proliferation,migration and chemotactic movement,ellagic acid can inhibit IL - 8 signal activation of prostate cancer cells,indicates the importance of ellagic acid inhibitor,prostate cancerin the prevention of prostate cancer recurrence and metastasis may have important role.
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