目的：研究华蟾素对人膀胱肿瘤T24细胞的作用及其机制。方法 MTT法研究华蟾素对细胞的增殖作用；将细胞分为空白对照、Caspase抑制剂、华蟾素、华蟾素+Caspase抑制剂4组，流式细胞术分析各组细胞凋亡及细胞周期，RT-qPCR法检测各组细胞AIF、Endo G mRNA表达情况。结果华蟾素能抑制T24细胞增殖；与空白组和Caspase抑制剂组相比，华蟾素组与华蟾素+Caspase抑制剂组细胞凋亡明显（P<0.05），细胞周期主要阻断在S期，并且两组细胞内AIF、Endo G mRNA表达明显上调（P<0.05）。结论华蟾素能抑制T24细胞增殖，通过Caspase依赖途径及Caspase非依赖途径诱导T24细胞凋亡。%Objective To explore the effect of Cinobuotalin on human bladder carcinoma cells and the underlining mechanism. Methods The Cinobuotalin’s promoting effect on cells was evaluated with MTT, the cells were divided into 4 groups: blank control, caspase inhabit, Cinobuotalin, Cinobuotalin+Caspase inhabit, the apoptosis and cell cycle were determined with flow cytometry, the expressions of AIF、Endo G mRNA were determined with RT-qPCR.Results Cinobuotalin inhabits the growth of T24, the apoptosis was significantly more in Cinobuotalin and Cinobuotalin+Caspase than in blank control and caspase inhabit groups(P<0.05). The cells were arrested mostly in S phase, the expression of AIF、Endo G mRNA were significantly higher in these two groups(P<0.05). Conclusion Cinobuotalin inhabits the growth of T24, which was achieved by inducing the apoptosis through the both the caspase depandant and caspase indepandant pathways.
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