目的探讨核转录因子家族中NF-Kb1基因-94ins/delATTG 的多态性与我国早发冠心病（早发CHD）的相关性。方法对早发冠心病（病例组）92例和非冠心病（对照组）94例患者，采用苯酚-氯仿法提取DNA，聚合酶链反应-限制性片段长度多态性方法（PCR-RFLP）分析NF-kappaB1基因多态性。结果两组病例del/del型纯合子，ins/ins型纯合子，del/ins型杂合子基因型频率及ins、del等位基因频率差异无统计学意义。结论 NF-kappaB1基因多态性与我国早发冠心病的易感性可能无关。%Objective To investigate the relationship between Nuclearfactor-kB(NF-kB)gene polymorphisms and premature coronary heart disease. Methods Ninty two patients of premature coronary heart diease and 94 controls were enrolled and polymerase chain reaction-restriction fragement length polymorphism(PCR-RFLP)was used to analysis,NF-kB1 gene polymorphism .Results There were no significant difference of the genotype distribution and alleles of gene between patients with premature coronary heart disease and controls(P>0.05). Conclution The Results suggest that NF-kB1 gene polymorphisms are not associated with an increased risk of premature coronary heart disease in China.
展开▼
