目的 研究免疫相关性全血细胞减少症(IRP)患者骨髓单个核细胞中CD28/B7家族共刺激分子mRNA表达情况,探讨CD28/B7家族共刺激分子在IRP发病机制中的作用.方法 采用骨髓单个核细胞分离及 RNA 提取等方法,用荧光定量PCR技术检测CD28/B7家族共刺激分子中 细胞毒性T淋巴细胞相关抗原4(CTLA-4)、CD28、CD80、CD86mRNA表达量,统计和分析IRP疾病组和缺铁性贫血(IDA)对照组间CD28/B7家族共刺激分子mRNA表达差异.结果 IRP和IDA 骨髓单个核细胞中CD28/B7家族共刺激分子mRNA的表达有差异,其中IRP 患者骨髓单个核细胞中的CTLA-4mRNA 表达量较IDA组明显减低(P0.05).结论 IRP患者骨髓单个核细胞中CD28/B7家族共刺激分子mRNA存在异常表达,其中CTLA-4的表达下调在IRP的发生发展中可能起到了重要的作用.%Objective To study the expression of CD28/B7 family costimulatory molecules mRNA in bone marrow mononuclear cells of patients with immune-related pancytopenia(IRP),and to explore the role of CD28/B7 family costimulatory molecules in the pathogenesis of IRP. Methods The expressions of cytotoxic T lymphocyte-associated antigen 4(CTLA-4),CD28,CD80 and CD86 mRNA in CD28/B7 family costimulatory molecules were detected by real-time PCR and RNA extraction. Differences in mRNA expression of CD28/B7 family costimulatory molecules were analyzed between the IRP disease group and the iron deficiency poor(IDA)control group. Results The mRNA expression of CD28/B7 family costimulatory molecules was different in IRP and IDA bone marrow mononuclear cells. The expression of CTLA-4 mRNA in bone marrow mononuclear cells of IRP patients was significantly lower than that in IDA group(P0.05). Conclusion The mRNA of CD28/B7 family costimulatory molecules is abnormally expressed in bone marrow mononuclear cells of IRP patients,and the down-regulation of CTLA-4 may play an important role in the development of IRP.
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