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首页> 美国卫生研究院文献>Acta Crystallographica Section F: Structural Biology and Crystallization Communications >Structural insights into RipC a putative citrate lyase β subunit from a Yersinia pestis virulence operon
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Structural insights into RipC a putative citrate lyase β subunit from a Yersinia pestis virulence operon

机译：对鼠疫耶尔森氏菌毒力操纵子推定的柠檬酸裂解酶β亚基RipC的结构见解

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摘要

Yersinia pestis remains a threat, with outbreaks of plague occurring in rural areas and its emergence as a weapon of bioterrorism; thus, an improved understanding of its various pathogenicity pathways is warranted. The rip (required for intracellular proliferation) virulence operon is required for Y. pestis survival in interferon-γ-treated macrophages and has been implicated in lowering macrophage-produced nitric oxide levels. RipC, one of three gene products from the rip operon, is annotated as a citrate lyase β subunit. Furthermore, the Y. pestis genome lacks genes that encode citrate lyase α and γ subunits, suggesting a unique functional role of RipC in the Y. pestis rip-mediated survival pathway. Here, the 2.45 Å resolution crystal structure of RipC revealed a homotrimer in which each monomer consists of a (β/α)8 TIM-barrel fold. Furthermore, the trimeric state was confirmed in solution by size-exclusion chromatography. Through sequence and structure comparisons with homologous proteins, it is proposed that RipC is a putative CoA- or CoA-derivative binding protein.
机译：鼠疫耶尔森菌仍然是一种威胁，农村地区爆发鼠疫，并成为生物恐怖主义的武器；因此，有必要进一步了解其各种致病性途径。 rip（胞内增殖所需）毒力操纵子是鼠疫杆菌在干扰素-γ处理的巨噬细胞中存活所必需的毒力操纵子，并且与降低巨噬细胞产生的一氧化氮水平有关。 RipC是来自rip操纵子的三种基因产物之一，被标注为柠檬酸裂合酶β亚基。此外，Y.pestis基因组缺乏编码柠檬酸裂合酶α和γ亚基的基因，这表明RipC在鼠疫杆菌裂口介导的生存途径中具有独特的功能。在这里，RipC的2.45Å分辨率晶体结构揭示了一种均三聚体，其中每个单体均由（β/α）8 TIM桶状折叠组成。此外，通过尺寸排阻色谱法确认了溶液中的三聚体状态。通过与同源蛋白的序列和结构比较，提出RipC是推定的CoA或CoA衍生物结合蛋白。

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