首页> 美国卫生研究院文献>Acta Crystallographica Section F: Structural Biology and Crystallization Communications >Complex assembly crystallization and preliminary X-ray crystallographic analysis of the chicken CD8αα–BF2*0401 complex
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Complex assembly crystallization and preliminary X-ray crystallographic analysis of the chicken CD8αα–BF2*0401 complex

机译:鸡CD8αα–BF2 * 0401配合物的配合物组装结晶和初步X射线晶体学分析

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摘要

In the process of antigen presentation, the MHCI–CD8 complex is important for immune signal transduction by the activation of cytotoxic T cells. Here, the expression, purification, crystallization and X-ray analysis of the complex of the chicken MHC class I molecule BF2*0401 and CD8αα (CD8αα–BF2*0401) are reported. This complex was verified by SDS–PAGE analysis of a CD8αα–BF2*0401 crystal, which showed three bands corresponding to the molecular weights of BF2*0401, β 2-microglobulin and CD8α, respectively. The crystal of CD8αα–BF2*0401 diffracted to 2.8 Å resolution and belonged to space group P21, with unit-cell parameters a = 90.6, b = 90.8, c = 94.9 Å, β = 98°. The Matthews coefficient and solvent content were calculated to be 2.88 Å3 Da−1 and ∼57.3%, respectively.
机译:在抗原呈递过程中,MHCI–CD8复合物通过激活细胞毒性T细胞对免疫信号转导很重要。在此,报道了鸡MHC I类分子BF2 * 0401和CD8αα(CD8αα–BF2 * 0401)的复合物的表达,纯化,结晶和X射线分析。通过CD8αα-BF2* 0401晶体的SDS-PAGE分析验证了该复合物,该晶体显示出三个谱带,分别对应于BF2 * 0401,β2-微球蛋白和CD8α的分子量。 CD8αα–BF2 * 0401晶体衍射至2.8Å的分辨率,属于P21空间群,其晶胞参数a = 90.6,b = 90.8,c = 94.9,β= 98°。马修斯系数和溶剂含量经计算分别为2.88Å 3 Da -1 和〜57.3%。

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