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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the chicken MHC class I molecule BF2*1501
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Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the chicken MHC class I molecule BF2*1501

机译：鸡MHC I类分子BF2 * 1501的复杂组装，结晶和初步X射线晶体学分析

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摘要

The chicken major histocompatibility complex (MHC) class I molecules named BFare strongly associated with Marek's disease (MD). A single structure, that of chicken BF2*2101 from the B21 haplotype, which might provide resistance to MD, has been determined. However, little is known about other structures apart from BF2*2101. In order to provide further structures of chicken MHC class I molecules, BF2*1501 and chicken beta(2)-microglobulin complexed with a nonapeptide (MDV-MEQ(RRR9)) derived from Marek's disease virus MEQ protein (MDV EcoRI Q fragment, residues 72-80) were assembled and crystallized. Diffraction data from the crystal were collected to 2.6 angstrom resolution; the crystal belonged to space group P3(1)21, with unit-cell parameters a = 125.1, b = 125.1, c = 80.9 angstrom and two molecules in the asymmetric unit. The Matthews coefficient V-M was 2.08 angstrom(3) Da(-1), with a calculated solvent content of 40.78%. These data will be helpful in obtaining insight into the structural basis of the involvement of BF2*1501 in MD.
机译：鸡的主要组织相容性复合体（MHC）I类分子BF与马立克氏病（MD）密切相关。已经确定了单个结构，即来自B21单倍型的BF2 * 2101鸡的结构，它可能对MD产生抗性。但是，除BF2 * 2101以外的其他结构知之甚少。为了提供鸡MHC I类分子的进一步结构，BF2 * 1501和鸡β（2）-微球蛋白与衍生自马立克氏病病毒MEQ蛋白（MDV EcoRI Q片段，残基）的九肽（MDV-MEQ（RRR9））复合72-80）组装并结晶。收集晶体的衍射数据至2.6埃分辨率;该晶体属于空间群P3（1）21，单位晶胞参数a = 125.1，b = 125.1，c = 80.9埃，两个分子在不对称单元中。马修斯系数V-M为2.08埃（3）Da（-1），计算出的溶剂含量为40.78％。这些数据将有助于深入了解BF2 * 1501参与MD的结构基础。

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