• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Regulation of aflR and Its Product, AflR, Associated with Aflatoxin Biosynthesis
【2h】

Regulation of aflR and Its Product, AflR, Associated with Aflatoxin Biosynthesis

机译：与黄曲霉毒素生物合成相关的aflR及其产物AflR的调控

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We studied the role of the regulatory gene aflR and its product, AflR, in the biosynthesis of aflatoxin in Aspergillus. Western blot and enzyme-linked immunosorbent assay analyses revealed that aflatoxin B1 accumulation was directly related to AflR expression and was regulated by various environmental and nutritional conditions, including temperature, air supply, carbon source, nitrogen source, and zinc availability. Expression of an aflatoxin biosynthetic pathway structural gene, omtA, was regulated by the presence of AflR. Induction patterns for aflR mRNA and AflR were correlated with that for omtA mRNA in an aflatoxin-producing strain of Aspergillus parasiticus. Analysis of non-aflatoxin-producing strains of A. flavus, A. sojae, and A. oryzae grown in medium suitable for aflatoxin B1 production showed that both aflR mRNA and AflR production were present; however, omtA mRNA production was not detected in any of these examined strains. AflR in the A. oryzae strain was regulated by carbon source and temperature in a manner similar to that seen with A. parasiticus.
机译：我们研究了调控基因aflR及其产物AflR在曲霉菌中黄曲霉毒素的生物合成中的作用。 Western印迹和酶联免疫吸附测定分析表明，黄曲霉毒素B1积累与AflR表达直接相关，并受多种环境和营养条件的调节，包括温度，空气供应，碳源，氮源和锌的可用性。黄曲霉毒素生物合成途径结构基因omtA的表达受AflR的存在调节。在产黄曲霉的寄生曲霉中，aflR mRNA和AflR的诱​​导模式与omtA mRNA的诱导模式相关。在适于黄曲霉毒素B1生产的培养基中生长的黄曲霉，大豆曲霉和米曲霉的非黄曲霉毒素生产菌株的分析表明，既存在aflR mRNA，又存在AflR。但是，在所有这些检测菌株中均未检测到omtA mRNA的产生。米曲霉菌株中的AflR受碳源和温度的调节，其方式类似于寄生寄生曲霉。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号