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首页> 美国卫生研究院文献>British Journal of Industrial Medicine >Enzyme immunoassays for total and allergen specific IgE in population studies.
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Enzyme immunoassays for total and allergen specific IgE in population studies.

机译：人群研究中针对总IgE和过敏原特异性IgE的酶免疫法。

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OBJECTIVE--Extensive IgE serology in occupational or environmental health studies is often hampered by a lack of technical facilities and finance. The use in population studies of relatively simple and inexpensive enzyme immunoassays (EIAs) was therefore evaluated for the assessment of total serum immunoglobulin E (IgE), and of specific IgE reactions with various common (house dust mites, grass and birch pollen, and cat) or occupational (fungal alpha-amylase and rat urinary protein) allergens. METHODS--Total IgE was measured with a sandwich EIA, calibrated with commercially available IgE standards. Reproducibility was studied by testing pooled normal human serum samples in each of a large series of test plates. A panel of 156 children's serum samples with known IgE values was used to compare the assay with other total IgE assays. A previously developed EIA for anti-yeast IgE was adapted for the measurement of IgE reacting with various common and occupational allergens. Binding of IgE to microwells coated with commercially available allergen extracts, or allergen preparations from our own laboratory, was measured with a monoclonal anti-human IgE antibody and subsequent incubations with biotinylated rabbit anti-mouse Ig and avidin-peroxidase. Panels of serum samples from school children (n = 116), bakery workers (n = 126), and laboratory animal workers (n = 52) were used to study sensitivity and specificity, with reference to skin prick tests as the standard, and to compare the EIAs with commercially available test kits. RESULTS--The detection limit of the EIA for total IgE was 0.5-1 kU/l for undiluted serum samples, and the coefficient of variation between assays was less than 15% at serum concentrations between 1 and 150 kU/l. Results obtained with the panel of 156 children's serum samples were strongly correlated (r2 = 0.86) with IgE concentrations measured previously by radioimmunoassay. The results of the EIA for various occupational allergens correlated very well, both qualitatively and quantitatively, with the results of commercial test kits. Sensitivity and specificity of the EIA results as a predictor of skin prick test reactivity towards common allergens (house dust mite, grass pollen, birch pollen, and cat) were remarkably high (> 80%-90%) in the series of 116 children's serum samples. In a population of bakery workers the specificity of the EIAs was also very high (> 90%). The sensitivity was notably lower (30%-70%) in this adult population, which is, however, in agreement with results reported for conventional IgE tests. CONCLUSION--As the costs were estimated to be at least five to 10-fold lower than those of commercial test kits, the EIAs for total and specific IgE may be very useful tools in epidemiological studies of atopic respiratory or other disorders.
机译：目的-在职业或环境健康研究中广泛的IgE血清学常常因缺乏技术设施和资金而受阻。因此，在人群研究中评估了相对简单和廉价的酶免疫测定法（EIA），以评估总血清免疫球蛋白E（IgE）以及与各种常见（屋尘螨，草和桦木花粉以及猫）的特定IgE反应的评估。 ）或职业性（真菌α-淀粉酶和大鼠尿蛋白）过敏原。方法-总IgE用三明治EIA进行测量，该EIA用市售IgE标准品校准。通过在大量测试板中的每组中测试合并的正常人血清样品来研究可重复性。使用一组156个IgE值已知的儿童血清样本，将其与其他总IgE测定进行比较。先前开发的针对抗酵母菌的IgE的EIA适于测量与各种常见和职业过敏原反应的IgE。用单克隆抗人IgE抗体测量IgE与涂有市售过敏原提取物或我们自己实验室的过敏原制剂包被的微孔的结合，然后与生物素化的兔抗小鼠Ig和抗生物素蛋白过氧化物酶一起孵育。参照皮肤点刺试验作为标准，从学童（n = 116），面包店工人（n = 126）和实验动物工人（n = 52）的血清样本小组中研究敏感性和特异性。将EIA与市售的测试套件进行比较。结果-未稀释血清样品的总IgE的EIA检出限为0.5-1 kU / l，在1至150 kU / l的血清浓度下，测定之间的变异系数小于15％。从156个儿童血清样本中获得的结果与先前通过放射免疫测定法测得的IgE浓度高度相关（r2 = 0.86）。各种职业变应原的EIA结果在质量和数量上与商业化试剂盒的结果非常相关。在116例儿童血清中，EIA结果的敏感性和特异性可作为皮肤点刺试验对常见过敏原（屋尘螨，草花粉，桦树花粉和猫）反应性的预测指标，非常高（> 80％-90％）样品。在面包店工人中，EIA的特异性也很高（> 90％）。在该成年人群中，敏感性显着降低（30％-70％），这与常规IgE测试报道的结果一致。结论-由于估计费用比商业检测试剂盒低至少五到十倍，因此总和特异性IgE的EIA可能是特应性呼吸道疾病或其他疾病流行病学研究中非常有用的工具。

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