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首页> 美国卫生研究院文献>Clinical and Diagnostic Laboratory Immunology >Development of Diagnostic Reagents To Differentiate between Mycobacterium bovis BCG Vaccination and M. bovis Infection in Cattle
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Development of Diagnostic Reagents To Differentiate between Mycobacterium bovis BCG Vaccination and M. bovis Infection in Cattle

机译：鉴别诊断试剂的开发 牛分枝杆菌卡介苗接种和M.之间 牛的牛感染

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In Great Britain a recent independent scientific review for the government has concluded that the development of a cattle vaccine against Mycobacterium bovis holds the best long-term prospect for tuberculosis control in British herds. A sine qua non for vaccination is the development of a complementary diagnostic test to differentiate between vaccinated animals and those infected with M. bovis so that test-and-slaughter-based control strategies can continue alongside vaccination. In order to assess the feasibility of developing a differential diagnostic test for a live vaccine, we chose M. bovis BCG Pasteur as a model system. Recombinant forms of antigens which are expressed in M. bovis but not, or only at low levels, in BCG Pasteur (ESAT-6, MPB64, MPB70, and MPB83) were produced. These reagents were tested either alone or in combination by using peripheral blood mononuclear cells from M. bovis-infected, BCG-vaccinated, and Mycobacterium avium-sensitized calves. All four antigens induced in vitro proliferation and gamma interferon responses only in M. bovis-infected animals. A cocktail composed of ESAT-6, MPB64, and MPB83 identified infected animals but not those vaccinated with BCG. In addition, promiscuous T-cell epitopes of ESAT-6, MPB64, and MPB83 were formulated into a peptide cocktail. In T-cell assays with this peptide cocktail, infected animals were identified with frequencies similar to those obtained in assays with the protein cocktail, while BCG-vaccinated or M. avium-sensitized animals did not respond. In summary, our results suggest that peptide and protein cocktails can be designed to discriminate between M. bovis infection and BCG vaccination.
机译：在英国，最近对政府进行的独立科学审查得出的结论是，针对牛分枝杆菌的牛疫苗的开发具有控制英国牛群结核病的最佳长期前景。接种疫苗的必要条件是开发一种互补的诊断测试，以区分接种疫苗的动物和感染牛分枝杆菌的动物，以便基于测试和屠宰的控制策略可以与接种疫苗一起继续。为了评估开发活疫苗差异诊断测试的可行性，我们选择了牛分枝杆菌BCG Pasteur作为模型系统。产生了在牛分枝杆菌中表达的抗原的重组形式，但在BCG巴斯德中不表达或仅以低水平表达（ESAT-6，MPB64，MPB70和MPB83）。使用来自牛分枝杆菌感染，BCG疫苗接种和鸟分枝杆菌致敏的牛的外周血单核细胞单独或组合测试这些试剂。所有四种抗原仅在体外诱导体外增殖和γ干扰素应答。 牛分枝杆菌感染的动物。由ESAT-6组成的鸡尾酒， MPB64和MPB83识别出感染的动物，但未接种疫苗 与BCG。此外，ESAT-6，MPB64， 将MPB83和MPB83配制成肽混合物。在T细胞分析中 通过这种肽混合物，可以确定感染动物 频率类似于使用该蛋白进行分析时获得的频率 鸡尾酒，同时接种BCG疫苗或致鸟分枝杆菌 动物没有反应。总而言之，我们的结果表明肽 蛋白质鸡尾酒可以用来区分M。 牛感染和卡介苗接种。

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