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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Development of Diagnostic Reagents To Differentiate between Mycobacterium bovis BCG Vaccination and M. bovis Infection in Cattle
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Development of Diagnostic Reagents To Differentiate between Mycobacterium bovis BCG Vaccination and M. bovis Infection in Cattle

机译：鉴别牛牛分枝杆菌卡介苗和牛分枝杆菌感染的诊断试剂的开发

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In Great Britain a recent independent scientific review for the government has concluded that the development of a cattle vaccine against Mycobacterium bovis holds the best long-term prospect for tuberculosis control in British herds. A sine qua non for vaccination is the development of a complementary diagnostic test to differentiate between vaccinated animals and those infected withM. bovis so that test-and-slaughter-based control strategies can continue alongside vaccination. In order to assess the feasibility of developing a differential diagnostic test for a live vaccine, we chose M. bovis BCG Pasteur as a model system. Recombinant forms of antigens which are expressed in M. bovis but not, or only at low levels, in BCG Pasteur (ESAT-6, MPB64, MPB70, and MPB83) were produced. These reagents were tested either alone or in combination by using peripheral blood mononuclear cells from M. bovis-infected, BCG-vaccinated, andMycobacterium avium-sensitized calves. All four antigens induced in vitro proliferation and gamma interferon responses only inM. bovis-infected animals. A cocktail composed of ESAT-6, MPB64, and MPB83 identified infected animals but not those vaccinated with BCG. In addition, promiscuous T-cell epitopes of ESAT-6, MPB64, and MPB83 were formulated into a peptide cocktail. In T-cell assays with this peptide cocktail, infected animals were identified with frequencies similar to those obtained in assays with the protein cocktail, while BCG-vaccinated or M. avium-sensitized animals did not respond. In summary, our results suggest that peptide and protein cocktails can be designed to discriminate between M. bovis infection and BCG vaccination.
机译：在英国，最近对政府进行的独立科学审查得出的结论是，针对牛分枝杆菌的牛疫苗的开发在英国人群中控制结核病具有最佳的长期前景。进行疫苗接种的必要条件是开发一种互补的诊断测试，以区分接种疫苗的动物和感染M的动物。 bovis ，因此基于测试和屠宰的控制策略可以与疫苗一起继续使用。为了评估开发活疫苗差异诊断测试的可行性，我们选择了 M。 bovis BCG Pasteur作为模型系统。在 M中表达的抗原的重组形式。在BCG巴斯德（ESAT-6，MPB64，MPB70和MPB83）中产生了牛黄体素，但没有或仅少量出现。这些试剂可以单独使用，也可以结合使用来自 M的外周血单个核细胞进行测试。牛感染，卡介苗接种和禽分枝杆菌致敏的犊牛。所有四种抗原仅在em中诱导体外增殖和γ干扰素应答。牛感染的动物。由ESAT-6，MPB64和MPB83组成的混合物可识别出感染的动物，但未发现接种过BCG的动物。此外，将ESAT-6，MPB64和MPB83的混杂T细胞表位配制为肽混合物。在使用这种肽混合物进行T细胞试验中，被感染的动物的频率与使用蛋白混合物进行免疫接种或Bem M时所鉴定的频率相似。鸟致敏的动物没有反应。总而言之，我们的结果表明可以设计肽和蛋白质混合物来区分 M。牛感染和卡介苗接种。

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