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首页> 美国卫生研究院文献>Clinical and Diagnostic Laboratory Immunology >Immunoglobulin M Antibody Responses to Mycobacterium ulcerans Allow Discrimination between Cases of Active Buruli Ulcer Disease and Matched Family Controls in Areas Where the Disease Is Endemic
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Immunoglobulin M Antibody Responses to Mycobacterium ulcerans Allow Discrimination between Cases of Active Buruli Ulcer Disease and Matched Family Controls in Areas Where the Disease Is Endemic

机译：对溃疡分枝杆菌的免疫球蛋白M抗体反应可区分活动性布鲁氏溃疡病病例和该病流行地区的配套家庭控制

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Buruli ulcer disease (BUD) is an emerging disease caused by Mycobacterium ulcerans. In the present study we have characterized the serological reactivities of sera from volunteer case patients with laboratory-confirmed BUD and controls living in three different regions of Ghana where the disease is endemic to determine if serology may be useful for disease confirmation. Our results showed highly reactive immunoglobulin G (IgG) responses among patients with laboratory-confirmed disease, healthy control family members of the case patients, and sera from patients with tuberculosis from areas where BUD is not endemic. These responses were represented by reactivities to multiple protein bands found in the M. ulcerans culture filtrate (CF). In contrast, patient IgM antibody responses to the M. ulcerans CF (MUCF) proteins were more distinct than those of healthy family members living in the same village. A total of 84.8% (56 of 66) of the BUD patients exhibited strong IgM antibody responses against MUCF proteins (30, 43 and 70 to 80 kDa), whereas only 4.5% (3 of 66) of the family controls exhibited such responses. The sensitivity of the total IgM response for the patients was 84.8% (95% confidence interval [CI], 74.3 to 91.6%), and the specificity determined with sera from family controls was 95.5% (95% CI, 87.5 to 98.4%). These studies suggest that the IgM responses of patients with BUD will be helpful in the identification and production of the M. ulcerans recombinant antigens required for the development of a sensitive and specific serological assay for the confirmation of active BUD.
机译：布鲁氏溃疡病（BUD）是一种由溃疡分枝杆菌引起的新兴疾病。在本研究中，我们已经表征了自愿者病例患者的血清学血清学反应性，这些患者具有实验室确认的BUD，并且生活在加纳的三个地方，这些地方是该病的地方性流行地区，以确定血清学是否可用于疾病确认。我们的结果显示，在实验室确诊的疾病患者，病例患者的健康对照家庭成员以及非BUD流行地区的结核病患者血清中，高反应性免疫球蛋白G（IgG）反应。这些反应以对溃疡分枝杆菌培养物滤液（CF）中发现的多个蛋白条带的反应性表示。相比之下，患者IgM对溃疡分枝杆菌CF（MUCF）蛋白的反应比住在同一村庄的健康家庭成员的反应更为明显。总共84.8％（66个中的56个）BUD患者表现出针对MUCF蛋白（30、43和70至80 kDa）的强IgM抗体反应，而家庭对照组中只有4.5％（66个中的3个）表现出这种反应。患者的总IgM反应敏感性为84.8％（95％置信区间[CI]为74.3至91.6％），用家庭对照组血清测定的特异性为95.5％（95％CI为87.5至98.4％）。 。这些研究表明，BUD患者的IgM反应将有助于鉴定和生产溃疡分枝杆菌重组抗原，从而开发出灵敏且特异的血清学检测方法来确认活动性BUD。

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