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首页> 美国卫生研究院文献>Clinical and Diagnostic Laboratory Immunology >T- and B-Cell Immune Responses of Patients Who Had Undergone Colectomies to Oral Administration of Salmonella enterica Serovar Typhi Ty21a Vaccine
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T- and B-Cell Immune Responses of Patients Who Had Undergone Colectomies to Oral Administration of Salmonella enterica Serovar Typhi Ty21a Vaccine

机译：接受过肠壁切除术的患者对肠炎沙门氏菌血清型伤寒Typhi Ty21a疫苗的口服T细胞和B细胞免疫反应

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The capacity of an oral live attenuated Salmonella enterica serovar Typhi Ty21a vaccine to induce immune responses in patients who had undergone colectomies because of ulcerative colitis was evaluated, and these responses were compared with those of healthy volunteers. Purified CD4+ and CD8+ T cells from peripheral blood were stimulated in vitro by using the heat-killed Ty21a vaccine strain, and the proliferation and gamma interferon (IFN-γ) production were measured before and 7 or 8 days after vaccination. Salmonella-specific immunoglobulin A (IgA) and IgG antibody responses in serum along with IgA antibody responses in ileostomy fluids from the patients who had undergone colectomies were also evaluated. Three doses of vaccine given 2 days apart failed to induce proliferative T-cell responses in all the six patients who had undergone colectomies, and increases in IFN-γ production were found only among the CD8+ cells from three of the patients. In contrast, both proliferative responses and increased IFN-γ production were observed among CD4+ and CD8+ T cells from 3 and 6 of 10 healthy volunteers, respectively. Salmonella-specific IgA and/or IgG antibody responses in serum were observed for five (56%) of nine patients who had undergone colectomies and in 15 (88%) of 17 healthy volunteers. In ileostomy fluids, significant anti-Salmonella IgA antibody titer increases were detected in six (67%) of nine patients who had undergone colectomies. The impaired T- and B-cell immune responses found after vaccination in the circulation of patients who have undergone colectomies may be explained by a diminished colonization of the Ty21a vaccine strain due to the lack of a terminal ileum and colon.
机译：评估了口服减毒活肠炎沙门氏菌伤寒Typhi Ty21a疫苗在因溃疡性结肠炎而接受肠壁切除术的患者中诱导免疫反应的能力，并将这些反应与健康志愿者进行了比较。用热灭活的Ty21a疫苗株体外刺激外周血中纯化的CD4 + 和CD8 + T细胞，并刺激其增殖和γ-干扰素（IFN-γ）。在疫苗接种之前和之后的7或8天测量产量。还评估了血清中的沙门氏菌特异性免疫球蛋白A（IgA）和IgG抗体反应，以及接受鞘膜切除术的患者回肠造口液中的IgA抗体反应。间隔2天服用三剂疫苗未能在所有接受鞘膜切除术的6例患者中诱导增生性T细胞反应，并且仅在3例CD8 + 细胞中发现IFN-γ产生增加的病人。相反，分别在10名健康志愿者中的3名和6名CD4 + 和CD8 + T细胞中观察到增殖反应和IFN-γ产生增加。在接受Colectomies的9例患者中有5例（56％）以及在17名健康志愿者中的15例（88％）中观察到血清中沙门氏菌特异性IgA和/或IgG抗体反应。在回肠造口液中，在接受结肠电切术的9例患者中，有6例（67％）检测到抗沙门氏菌IgA抗体效价显着增加。接受colectomies的患者循环中发现疫苗接种后发现的T细胞和B细胞免疫反应受损，可能是由于缺乏回肠和结肠末端而导致Ty21a疫苗株的定殖减少。

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